PMID: 4290533Jul 1, 1966Paper

The kinetics and mechanism of liver alcohol dehydrogenase with primary and secondary alcohols as substrates

The Biochemical Journal
K Dalziel, F M Dickinson

Abstract

1. The activity of liver alcohol dehydrogenase with propan-2-ol and butan-2-ol has been confirmed. The activity with the corresponding ketones is small. Initial-rate parameters are reported for the oxidation of these secondary alcohols, and of propan-1-ol and 2-methylpropan-1-ol, and for the reduction of propionaldehyde and 2-methylpropionaldehyde. Substrate inhibition with primary alcohols is also described. 2. The requirements of the Theorell-Chance mechanism are satisfied by the data for all the primary alcohols and aldehydes, but not by the data for the secondary alcohols. A mechanism that provides for dissociation of either coenzyme or substrate from the reactive ternary complex is described, and shown to account for the initial-rate data for both primary and secondary alcohols, and for isotope-exchange results for the former. With primary alcohols, the rapid rate of reaction of the ternary complex, and its small steady-state concentration, result in conformity of initial-rate data to the requirements of the Theorell-Chance mechanisms. With secondary alcohols, the ternary complex reacts more slowly, its steady-state concentration is greater, and therefore dissociation of coenzyme from it is rate-limiting with non-saturatin...Continue Reading

Citations

Jan 1, 1994·Critical Reviews in Microbiology·M F Reid, C A Fewson
May 13, 2014·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·R J SlaughterL J Schep
Nov 7, 1972·European Journal of Biochemistry·I Björkhem
May 1, 1970·European Journal of Biochemistry·C H ReynoldsJ S McKinley-McKee
Mar 23, 2011·Neurochemistry International·Paul C Engel
Oct 15, 1984·European Journal of Biochemistry·P AnderssonG Pettersson
Mar 15, 1984·European Journal of Biochemistry·P AnderssonG Pettersson
Apr 18, 2001·Chemico-biological Interactions·J BenachR Gonzàlez-Duarte
Jan 1, 1983·Pharmacology, Biochemistry, and Behavior·W F BosronT K Li
Jan 1, 1983·Pharmacology, Biochemistry, and Behavior·T Cronholm
Dec 21, 2002·European Journal of Biochemistry·Marie TrovasletMarie Dominique Legoy
Jan 1, 1985·Reviews on Drug Metabolism and Drug Interactions·K E Crow
Feb 1, 1974·British Journal of Pharmacology·M M AsaadB N Dixit
Jan 11, 1977·Biochemistry·R T Dworschack, B V Plapp
Oct 14, 1980·Biochemistry·C N Ryzewski, R Pietruszko
Jan 1, 1973·Experimental Eye Research·R Vento, F Cacioppo
Jul 1, 1993·Biotechnology and Bioengineering·J B FordK B Taylor
Jun 15, 1978·Journal of Molecular Biology·B V PlappC I Brändén
Jul 7, 2017·Chemical Communications : Chem Comm·João C P de SouzaFrank N Crespilho
Apr 1, 2008·Human & Experimental Toxicology·M VujasinovicM Brvar
Sep 2, 2000·Journal of Biochemical and Molecular Toxicology·B F DudleyG W Winston
Apr 15, 2000·The Journal of Biological Chemistry·H A Charlier, B V Plapp
Jul 27, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hidehiko HirakawaTeruyuki Nagamune

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.