The kinetics of serum-induced initiation of DNA synthesis in BHK 21/C13 cells, and the influence of exogenous adenosine

Journal of Cellular Physiology
R F Brooks

Abstract

After the re-addition of serum in the presence of adenosine (25 muM), the entry of quiescent, serum starved BHK 21 cells into DNA synthesis follows first order kinetics after a well defined lag period of eight hours, and with a rate constant dependent on serum concentration. Initiation of DNA synthesis under these conditions can therefore be considered to be a random event occurring with a "Transition Probability" determined by the serum concentration. In the presence of adenosine, the change of Transition Probability following the addition of serum occurs abruptly. In the absence of exogenous adenosine, however, the change of Transition Probability after serum addition appears to be both gradual and bi-phasic. The initial changes in the absence of adenosine, though smaller in magnitude, display a similar dependence on serum concentration to the changes occurring in the presence of the nucleoside. In contrast, the secondary gradual increase of Transition Probability in the absence of added purines exhibits a higher serum requirement. It is suggested that the regulation of Transition Probability by serum involves some purine-dependent process, and that in the absence of an exogenous supply this becomes limited by endogenous synt...Continue Reading

References

Feb 1, 1973·Journal of Cellular Physiology·G D Clarke, C Smith
Apr 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J A Smith, L Martin
Oct 1, 1971·Journal of Cellular Physiology·H M Temin
May 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·R W Holley, J A Kiernan
Dec 1, 1970·Experimental Cell Research·R R Bürk
Mar 1, 1960·Proceedings of the National Academy of Sciences of the United States of America·M Vogt, R Dulbecco
Dec 10, 1966·Nature·R R Bürk

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Citations

Jan 1, 1977·Journal of Supramolecular Structure·G S Barsh, D D Cunningham
Aug 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·L E LeeN C Bols
Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·E Rapaport, P C Zamecnik
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J CampisiA B Pardee
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R G Croy, A B Pardee
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R PepperkokC Schneider
Jan 1, 1993·Cell Proliferation·J E Souren, R van Wijk
Jul 1, 1988·Journal of Cellular Physiology·R M Böhmer, L D Beattie
Apr 1, 1981·Journal of Cellular Physiology·O W RønningP O Seglen
Nov 1, 1987·Cell and Tissue Kinetics·D L Dewey
Oct 1, 1978·Experimental Cell Research·A Yen, A B Pardee
Jul 1, 1981·Experimental Cell Research·O W Rønning, T Lindmo
Dec 21, 1977·Journal of Theoretical Biology·L Alberghina
Mar 18, 1976·Nature·R F Brooks
Feb 1, 1982·The Journal of Pathology·J A Smith
Jun 1, 1977·Cancer·A B Pardee, R Dubrow
Nov 1, 1982·Journal of Cellular Physiology·M LubinB A Coutermarsh
Apr 1, 1985·Journal of Cellular Physiology·C D ScherW J Pledger
Feb 1, 1977·Journal of Cellular Physiology·D C Dooley, H L Ozer
Dec 1, 1984·Journal of Cellular Physiology·K M RichmondR F Brooks
Dec 1, 1978·Journal of Cellular Physiology·A Talavera, C Basilico

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