The kinetochore proteins CENP-E and CENP-F directly and specifically interact with distinct BUB mitotic checkpoint Ser/Thr kinases

The Journal of Biological Chemistry
Giuseppe CiossaniAndrea Musacchio

Abstract

The segregation of chromosomes during cell division relies on the function of the kinetochores, protein complexes that physically connect chromosomes with microtubules of the spindle. The metazoan proteins, centromere protein E (CENP-E) and CENP-F, are components of a fibrous layer of mitotic kinetochores named the corona. Several of their features suggest that CENP-E and CENP-F are paralogs: they are very large (comprising ∼2700 and 3200 residues, respectively), contain abundant predicted coiled-coil structures, are C-terminally prenylated, and are endowed with microtubule-binding sites at their termini. Moreover, CENP-E contains an ATP-hydrolyzing motor domain that promotes microtubule plus end-directed motion. Here, we show that both CENP-E and CENP-F are recruited to mitotic kinetochores independently of the main corona constituent, the Rod/Zwilch/ZW10 (RZZ) complex. We identified specific interactions of CENP-F and CENP-E with budding uninhibited by benzimidazole 1 (BUB1) and BUB1-related (BUBR1) mitotic checkpoint Ser/Thr kinases, respectively, paralogous proteins involved in mitotic checkpoint control and chromosome alignment. Whereas BUBR1 was dispensable for kinetochore localization of CENP-E, BUB1 was stringently requ...Continue Reading

References

May 24, 1991·Science·A LupasJ Stock
Mar 1, 1991·Chromosoma·W C Earnshaw, R L Bernat
Jul 1, 1967·Journal of Ultrastructure Research·P T Jokelainen
Jan 1, 1993·Cell Motility and the Cytoskeleton·J B RattnerT J Yen
Feb 12, 1998·The Journal of Cell Biology·B T SchaarT J Yen
Aug 12, 1998·The Journal of Cell Biology·D A StarrM L Goldberg
Jan 9, 2001·Nature Cell Biology·R BastoR E Karess
May 23, 2002·Bioinformatics·Mauro Delorenzi, Terry Speed
Jan 9, 2004·Current Biology : CB·Renata BastoRoger Karess
Mar 17, 2004·Journal of Cell Science·Victoria L JohnsonStephen S Taylor
Dec 14, 2004·Nature Cell Biology·Michael A Lampson, Tarun M Kapoor
Apr 13, 2005·The Journal of Cell Biology·Geert J P L KopsDon W Cleveland
May 12, 2005·Current Biology : CB·Eulalie BuffinRoger E Karess
Jun 28, 2005·Nucleic Acids Research·Markus GruberAndrei N Lupas
Sep 8, 2005·The Journal of Cell Biology·Yinghui MaoDon W Cleveland
Jan 21, 2006·Science·Tarun M KapoorAlexey Khodjakov
Jun 30, 2007·Current Biology : CB·Maïlys A S Vergnolle, Stephen S Taylor

❮ Previous
Next ❯

Citations

Sep 4, 2019·Cell Cycle·Cheng-Jie ZhouCheng-Guang Liang
Mar 25, 2020·The Journal of Cell Biology·Philip AucklandAndrew D McAinsh
Apr 30, 2020·Essays in Biochemistry·Benjamin Craske, Julie P I Welburn
Jul 16, 2020·Journal of Cell Science·Thibault LegalJulie P I Welburn
May 18, 2020·Nature Communications·Peter A JohanssonNicholas K Hayward
Sep 11, 2018·Cell Cycle·Alessandro Berto, Valérie Doye
Mar 16, 2021·Animal Cells and Systems·Taekyung Kim, Anton Gartner
Sep 19, 2019·Biochemical and Biophysical Research Communications·Masayoshi YamaneAkira Asai
May 6, 2021·Brain Sciences·Nikistratos SiskosMaria E Grigoriou

❮ Previous
Next ❯

Methods Mentioned

BETA
two-hybrid
Y2H
size-exclusion chromatography
electron microscopy
dissection
PCR
transfection

Software Mentioned

Slidebook
SEDNTERP
GraphPad
Imaris
Marcoils
Adobe
Coils
Bitplane
GraphPad Prism
Pcoils

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.