PMID: 2987645Jan 1, 1985Paper

The Klebsiella aerogenes glutamate dehydrogenase (gdhA) gene: cloning, high-level expression and hybrid enzyme formation in Escherichia coli

Molecular & General Genetics : MGG
A MountainJ C Wootton

Abstract

The NADP-dependent glutamate dehydrogenase gene of Klebsiella aerogenes was cloned in E. coli in the expression plasmid pRK9. The cloned gene shows a high level of expression in E. coli in the hybrid plasmid pKG3 and such expression is independent of the vector promoter, as shown by experiments in which the promoter was deleted. Active hybrid GDH hexamers were shown in cell-free extracts of an E. coli strain carrying cloned gdhA genes of both E. coli and K. aerogenes. The nucleotide sequence of the N-terminal coding region of the K. aerogenes gdhA gene was determined and found to be strongly homologous with that of E. coli.

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Citations

Aug 1, 1990·Biochemical Genetics·D Papadopoulou, C Louis
Jul 1, 1987·Journal of Neurochemistry·C BannerR J Wenthold
Sep 11, 2010·Microbiology·Takeo TomitaMakoto Nishiyama

Related Concepts

DNA Sequence
Genome Mapping
DNA Restriction Enzymes
Alkalescens-Dispar Group
Cistron
Genes, Bacterial
Glutamate Dehydrogenase
Klebsiella rhinoscleromatis
Neurospora crassa
Macromolecular Compounds

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