The Kluyveromyces lactis alpha1,6-mannosyltransferase KlOch1p is required for cell-wall organization and proper functioning of the secretory pathway

FEMS Yeast Research
Daniela UccellettiClaudio Palleschi

Abstract

Mutants of Kluyveromyces lactis denominated vga (vanadate glycosylation affected) bear various combinations of glycosylation and cell-wall defects. The vga3 mutation of K. lactis was mapped in the KlOCH1 gene, encoding the functional homologue of the Saccharomyces cerevisiaealpha1,6-mannosyltransferase. Quantitative analysis of cell-wall components indicated a noticeable increase of chitin and beta1,6-glucans and a severe decrease of mannoproteins in the mutant cells as compared with the wild-type counterparts. Fine-structure determination of the beta1,6-glucan polymer indicated that, in the vga3-1 strain, the beta1,6-glucans are shorter and have more branches than in the wild-type strain. This suggests that cell-wall remodelling changes take place in K. lactis in the presence of glycosylation defects. Moreover, the vga3 cells showed a significantly improved capability of secreting heterologous proteins. Such a capability, accompanied by the highly reduced N-glycosylation, may be of biotechnological interest, especially when hyper-glycosylation of recombinant products must be avoided.

References

Apr 27, 1987·Biochimica Et Biophysica Acta·W Tanner, L Lehle
Feb 1, 1994·Molecular and Cellular Biology·Z HongB DiDomenico
Aug 23, 2000·Yeast·D UccellettiC Palleschi
Sep 1, 2001·Cellular and Molecular Life Sciences : CMLS·H Schachter
Mar 7, 2002·Applied Microbiology and Biotechnology·W Panuwatsuk, N A Da Silva
Jun 1, 2002·Microbiology and Molecular Biology Reviews : MMBR·Stefan Hohmann
May 8, 2003·Yeast·Anja LorbergJürgen J Heinisch
Apr 6, 2004·Journal of Biotechnology·Annamaria MericoConcetta Compagno

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Citations

Jun 30, 2010·Applied Microbiology and Biotechnology·Karen De PourcqNico Callewaert
Oct 7, 2008·Applied and Environmental Microbiology·S RaimondiD Uccelletti
Mar 8, 2008·Bioscience, Biotechnology, and Biochemistry·Yoshifumi Jigami
Oct 16, 2007·Current Opinion in Chemical Biology·Yasunori Chiba, Yoshifumi Jigami
Jan 13, 2015·Research in Microbiology·Elena ZanniDaniela Uccelletti
Apr 12, 2007·FEMS Yeast Research·Daniela UccellettiClaudio Palleschi
Oct 12, 2018·The Journal of General and Applied Microbiology·Ronilo Jose D FloresKazuhito Fujiyama
May 2, 2018·Microorganisms·Antonio Milton Vieira GomesNádia Skorupa Parachin

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