The Kv channel blocker 4-aminopyridine enhances Ag+ uptake: a scanning electrochemical microscopy study of single living cells.

Proceedings of the National Academy of Sciences of the United States of America
Dongping ZhanAllen J Bard

Abstract

We report that silver ion (Ag(+)) uptake is enhanced by 4-aminopyridine (4-AP), a well known voltage-sensitive potassium ion channel (K(v)) blocker. Both bacterial (Escherichia coli) and mammalian (3T3 fibroblast) cells were used as model systems. Ag(+) uptake was monitored with a scanning electrochemical microscope with an amperometric Ag(+) ion-selective electrode (Ag(+)-ISE) and the respiration rates of E. coli cells were measured by oxygen reduction at an ultramicroelectrode. The results showed that not only the amount but also the rate of silver uptake by the cells increased significantly when 4-AP was added to the solution. For fibroblasts, the Ag(+) uptake rate was 4.8 x 10(7) ions per cell per sec without 4-AP compared with 1.0 x 10(8) ions per cell per sec with 0.2 mM 4-AP. For E. coli cells, the uptake rate was 1.5 x 10(4) ions per cell per sec without 4-AP vs. 3.5 x 10(4) ions per cell per sec with 0.5 mM 4-AP and 5.9 x 10(4) ions per cell per sec with 1 mM 4-AP. Thus, 4-AP might be useful where silver is used as antimicrobial agent to speed its uptake.

References

Aug 1, 1979·Canadian Journal of Microbiology·A T Hendry, I O Stewart
Feb 1, 1992·The Journal of General Physiology·D Choquet, H Korn
Jun 1, 1991·European Journal of Biochemistry·V A KostyrkoM L Verkhovskaya
Feb 1, 1991·The Journal of Physiology·J R Howe, J M Ritchie
Jun 1, 1974·Canadian Journal of Microbiology·P D Bragg, D J Rainnie
Oct 1, 1982·Journal of Bacteriology·W J Schreurs, H Rosenberg
Apr 21, 1995·The Journal of Biological Chemistry·M Solioz, A Odermatt
Jan 1, 1994·Progress in Medicinal Chemistry·A D Russell, W B Hugo
Jan 1, 1994·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·L M Deshpande, B A Chopade
Jan 1, 1996·Annual Review of Microbiology·S Silver, L T Phung
Oct 27, 1998·Toxicology Letters·E Hidalgo, C Domínguez
Nov 24, 1999·The American Journal of Physiology·N R Bury, C M Wood
Jul 18, 2002·Antimicrobial Agents and Chemotherapy·Pavel DibrovClaudia C Häse
Mar 17, 2004·Burns : Journal of the International Society for Burn Injuries·Vincent K M Poon, Andrew Burd
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Janine Mauzeroll, Allen J Bard
Jul 16, 2004·Analytical Chemistry·Dongping ZhanYuanhua Shao
Dec 17, 2004·Proceedings of the National Academy of Sciences of the United States of America·Janine MauzerollTerrence J Monks
Feb 14, 2006·Pharmacology & Therapeutics·Susan I V Judge, Christopher T Bever
Jun 27, 2006·Biosensors & Bioelectronics·Allen J BardWei Zhan
Jul 21, 2006·Analytical and Bioanalytical Chemistry·Shigeru AmemiyaDarrick A Gross

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Citations

Oct 3, 2012·Analytical Chemistry·Dongping ZhanZhong-Qun Tian
Feb 2, 2011·Proceedings of the National Academy of Sciences of the United States of America·Xiuhui LiuAllen J Bard
Jul 20, 2010·Annual Review of Analytical Chemistry·Albert SchulteWolfgang Schuhmann
Mar 8, 2012·Bioanalysis·Yong LuoNing Fang
Jun 24, 2010·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Jesse BertinatoLouise J Plouffe
Sep 8, 2009·Free Radical Biology & Medicine·Miriam M Cortese-KrottChristoph V Suschek
Apr 15, 2015·The Analyst·Carlos Duarte-GuevaraRashid Bashir
Oct 17, 2018·The Analyst·Fraser P Filice, Zhifeng Ding
Mar 26, 2013·PloS One·Moritz Hetzer, Didier Sornette
Mar 11, 2011·Chemical Society Reviews·Shujuan LiuYuanhua Shao
Jul 22, 2020·Analytical and Bioanalytical Chemistry·Giada Caniglia, Christine Kranz

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