PMID: 8981261Oct 1, 1996Paper

The L-type Ca2+ channel is involved in microvesicle-mediated glutamate exocytosis from rat pinealocytes

Journal of Pineal Research
H YamadaY Moriyama

Abstract

Pinealocytes, parenchymal cells of the pineal gland, secrete glutamate through microvesicle-mediated exocytosis upon depolarization by KCl in the presence of Ca2+, which is involved in a novel paracrine-like intercellular signal transduction mechanism in neuroendocrine organs. In the present study, we investigated whether or not the L-type Ca2+ channel is involved in the microvesicle-mediated glutamate secretion from cultured rat pinealocytes. Nifedipine, a specific antagonist of the L-type Ca2+ channel, inhibited the Ca(2+)-dependent glutamate exocytosis by 48% at 20 microM. Other L-type Ca2+ channel antagonists, such as nitrendipine, showed similar effects. 1,4-Dihydro-2,6-dimethyl-5-nitro-4 [2-(trifluoromethyl)-phenyl]-3-pyridinecarboxylic acid methyl ester (BAY K8644), an agonist of the L-type Ca2+ channel, at 1 microM, on the other hand, stimulated the glutamate exocytosis about 1.6-fold. Consistently, these Ca2+ channel antagonists inhibited about 50% of the Ca2+ uptake, whereas BAY K8644 increased the uptake 5.3-fold. An antibody against the carboxyl-terminal region of the rabbit L-type Ca2+ channel recognized polypeptides of pinealocytes with apparent molecular masses of 250 and 270 kDa, respectively, and immunostained ...Continue Reading

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Citations

Jun 6, 1997·Neuroscience Letters·H YamadaY Moriyama
Jul 31, 1998·Neuroscience Letters·S IshioY Moriyama
Oct 24, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mean-Hwan KimDuk-Su Koh
Dec 17, 2014·The Journal of Physiology·Haijie YuBertil Hille
Dec 11, 2007·Neuroscience Research·Chiaki Fukuhara, Gianluca Tosini
Sep 7, 2005·Cell Biochemistry and Function·Solange C AfecheJosé Cipolla-Neto
Mar 29, 2016·Journal of Pineal Research·Haijie YuBertil Hille
Feb 21, 2014·American Journal of Physiology. Cell Physiology·Jin-Young YoonDuk-Su Koh
Jul 13, 2004·Molecular Pharmacology·Susan C HernandezKenneth J Kellar
Apr 18, 2020·Frontiers in Neuroscience·Susannah C LumsdenYusuf Ozgur Cakmak
Dec 23, 2003·European Journal of Biochemistry·Eiichi HinoiYukio Yoneda

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