PMID: 7518914Jul 11, 1994Paper

The La antigen inhibits the activation of the interferon-inducible protein kinase PKR by sequestering and unwinding double-stranded RNA

Nucleic Acids Research
Q XiaoM J Clemens

Abstract

The La (SS-B) autoimmune antigen is an RNA-binding protein that is present in both nucleus and cytoplasm of eukaryotic cells. The spectrum of RNAs that interact with the La antigen includes species which also bind to the interferon-inducible protein kinase PKR. We have investigated whether the La antigen can regulate the activity of PKR and have observed that both the autophosphorylation of the protein kinase that accompanies its activation by dsRNA and the dsRNA-dependent phosphorylation of the alpha subunit of polypeptide chain initiation factor eIF-2 by PKR are inhibited in the presence of recombinant La antigen. This inhibition is partially relieved at higher concentrations of dsRNA. Once activated by dsRNA the protein kinase activity of PKR is insensitive to the La antigen. We have demonstrated by a filter binding assay that La is a dsRNA binding protein. Furthermore, when recombinant La is incubated with a 900 bp synthetic dsRNA or with naturally occurring reovirus dsRNA it converts these substrates to single-stranded forms. We conclude that the La antigen inhibits the dsRNA-dependent activation of PKR by binding and unwinding dsRNA and that it may therefore play a role in the regulation of this protein kinase in interfer...Continue Reading

References

Apr 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·M J ClemensI M London
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M SchwemmleM Bachmann
Oct 28, 1992·Annals of the New York Academy of Sciences·K Nishikura
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H W ChangB L Jacobs
Dec 1, 1992·Genes & Development·S R Green, M B Mathews
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D St JohnstonM Jantsch
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S BlumH Trachsel
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T G LeeM G Katze
Oct 11, 1991·Nucleic Acids Research·G J PruijnW J van Venrooij
Jan 1, 1991·Annual Review of Biochemistry·J W Hershey
Jan 1, 1990·Molecular Biology Reports·G J PruijnW J Van Venrooij
Jan 1, 1987·Annual Review of Biochemistry·S PestkaC E Samuel
Feb 21, 1989·Molecular and Cellular Biochemistry·M BachmannW E Müller
Jan 2, 1989·European Journal of Biochemistry·J GalabruA G Hovanessian
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A M Francoeur, M B Mathews
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J WiluszJ D Keene
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·E F MeursA G Hovanessian
Feb 1, 1993·Molecular Biology Reports·M J Clemens

❮ Previous
Next ❯

Citations

Dec 1, 1995·Current Opinion in Immunology·W J van Venrooij, G J Pruijn
Jan 12, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Takeo ShimazakiKenichi Kobayashi
Oct 23, 1997·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M J Clemens, A Elia
Jan 15, 1997·Nucleic Acids Research·P HühnM Bachmann
Oct 22, 2008·Molecular and Cellular Biology·Aïcha DaherAnne Gatignol
Apr 1, 2006·Critical Reviews in Clinical Laboratory Sciences·John G RoutsiasAthanasios G Tzioufas
Mar 15, 1996·European Journal of Biochemistry·V M Pain
Mar 1, 1996·European Journal of Biochemistry·R PeekW J Van Venrooij
May 7, 2004·European Journal of Biochemistry·Androulla EliaMichael J Clemens
Apr 25, 2006·Biochemical and Biophysical Research Communications·Massimo SquatritoGiulio F Draetta
May 20, 2015·Biochimie·Konstantina KatsarouKriton Kalantidis
Apr 15, 2014·Virology·Shilei DingChen Liang
May 19, 2005·Cell Death and Differentiation·S J MorleyM J Clemens
Jan 22, 2019·Critical Reviews in Microbiology·Wen MengHwa-Chain Robert Wang
Nov 18, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Ladomery
Jul 21, 2020·RNA Biology·Gunhild Sommer, Tilman Heise
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·M Kumar, G G Carmichael

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.