The La autoantigen contains a dimerization domain that is essential for enhancing translation.

Molecular and Cellular Biology
A W CraigN Sonenberg

Abstract

The La autoantigen is an RNA-binding protein that is involved in initiation and termination of RNA polymerase III transcription. It also binds several viral RNAs, including those of poliovirus and human immunodeficiency virus (HIV). Binding of the La protein to these RNAs enhances their translation in vitro (K. Meerovitch, Y.V. Svitkin, H.S. Lee, F. Lejbkowicz, D.J. Kenan, E.K.L. Chan, V.L. Agol, J.D. Keene, and N. Sonenberg, J. Virol. 67:3798-3807, 1993, and Y.V. Svitkin, A. Pause, and N. Sonenberg, J. Virol. 68:7001-7007, 1994). Here, a functional domain in the carboxy-terminal half of La that is distinct from the RNA-binding domain is described. Deletion of this domain abrogated the ability of La protein to enhance translation of poliovirus RNA and a hybrid HIV trans-activation-response element-chloramphenicol acetyltransferase mRNA. Far-Western assays indicated that the La protein homodimerized in vitro, and the C-terminal deletions that caused a loss of activity in translation also abrogated the dimerization signal. Gel filtration chromatography of recombinant La protein confirmed that La protein exists as a dimer under native conditions. Addition of the purified dimerization domain resulted in a loss of translation stimul...Continue Reading

References

Aug 25, 1992·Nucleic Acids Research·A P Geballe, M K Gray
Nov 11, 1992·Nucleic Acids Research·K MarelloJ Sommerville
Jun 1, 1991·Trends in Biochemical Sciences·D J KenanJ D Keene
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D N SenGuptaR H Silverman
Oct 1, 1989·Current Opinion in Rheumatology·E K Chan, E M Tan
Sep 8, 1995·The Journal of Biological Chemistry·D Herschlag
Oct 6, 1995·Journal of Molecular Biology·V TanchouJ L Darlix
Jan 1, 1994·Annual Review of Biochemistry·K A Jones, B M Peterlin
Feb 1, 1996·Journal of Virology·N Pardigon, J H Strauss
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·R J Maraia

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Citations

Nov 17, 1998·The Journal of Cell Biology·J S RosenblumG Blobel
Dec 25, 2009·Journal of Autoimmunity·Markus H HoffmannGünter Steiner
May 15, 2009·Biochimica Et Biophysica Acta·Michael Niepmann
Oct 20, 2012·Virus Research·Sylvain de BreyneThéophile Ohlmann
Mar 7, 2003·Cancer Cell·Rossana TrottaBruno Calabretta
May 30, 2008·Journal of Molecular Biology·Witold SzaflarskiKnud H Nierhaus
Dec 19, 2014·Nucleic Acids Research·Julia KuehnertTilman Heise
Jun 5, 2002·Annual Review of Biochemistry·Sandra L Wolin, Tommy Cedervall
Oct 20, 1998·DNA and Cell Biology·J KremerskothenJ Brosius
Mar 29, 2002·Arthritis and Rheumatism·Theophany EystathioyEdward K L Chan
Jan 8, 2013·Future Virology·Amanda J Chase, Bert L Semler
Aug 7, 2017·Wiley Interdisciplinary Reviews. RNA·Richard J MaraiaMaria R Conte
Nov 5, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J C LabbéL A Rokeach
May 9, 2001·The Journal of Biological Chemistry·U M OhndorfP Sondermann
Apr 27, 2000·The Journal of Biological Chemistry·Y Ueda, G Chaudhuri
Jul 5, 2001·Molecular and Cellular Biology·K KhaleghpourN Sonenberg
Jul 21, 2020·RNA Biology·Gunhild Sommer, Tilman Heise
Sep 1, 2014·Translation·Théophile OhlmannMarcelo López-Lastra
Sep 6, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M F Wilkinson, A B Shyu
Jul 18, 2002·The Journal of Biological Chemistry·Sven HorkeTilman Heise
Jan 2, 2019·International Journal of Molecular Sciences·Sylvain de Breyne, Théophile Ohlmann
May 26, 2020·Biochimica Et Biophysica Acta. Gene Regulatory Mechanisms·Aldo BarreraMarcelo López-Lastra

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