PMID: 1195788May 1, 1975Paper

The lack of altered enzyme molecules in "senescent" mouse embryo fibroblasts in culture

Mechanisms of Ageing and Development
M Danot, H Gershon

Abstract

In order to compare the biochemical events occurring in senescent animals in vivo with those of fibroblasts demonstrating a limited life span in tissue culture, experiments were performed to determine whether altered enzyme molecules are produced in late passage mouse embryo fibroblasts in tissue culture. Such altered enzymes have been previously described by us in both senescent mice and nematodes. Fibroblast cultures, initiated from BALB/c embryos underwent approximately 7 population doublings before reaching stationary phase. A comparison was made of the enzyme activity and the quantity of antigen of the enzyme aldolase present in these cultures. The amound of aldolase per cell as well as the amount of enzyme per unit of antigen remained constant throughout the in vitro lifespan of these cells. Further evidence testifying to a lack of enzyme alteration in vitro was obtained in experiments testing temperature sensitivity. The aldolase from all passages was equally sensitive to heating at 60 degrees C.

References

Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H Gershon, D Gershon
Apr 1, 1973·Mechanisms of Ageing and Development·H Gershon, D Gershon
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·S A AaronsonG J Todaro
Feb 1, 1970·Archives of Biochemistry and Biophysics·R W GracyB L Horecker
Jun 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·G A TomkinsL Hayflick
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·L Packer, J R Smith
Feb 1, 1972·Experimental Cell Research·L Lima, A Macieira-Coelho
Sep 19, 1970·Nature·H Gershon, D Gershon
Nov 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·L E Orgel
Oct 6, 1972·Nature·C M Lewis, G M Tarrant
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Mar 1, 1913·The Journal of Experimental Medicine·A H Ebeling

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Citations

Jul 1, 1977·Mechanisms of Ageing and Development·M Rothstein
Jul 1, 1978·Mechanisms of Ageing and Development·D Viceps-Madore, V J Cristofalo
Oct 1, 1979·Mechanisms of Ageing and Development·A L Burns, M S Kaulenas
Mar 1, 1987·Mechanisms of Ageing and Development·B M Stanulis-Praeger
Aug 1, 1983·Biological Reviews of the Cambridge Philosophical Society·S C Makrides
Feb 1, 1978·Journal of Cellular Physiology·A KaftoryM Fry

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