The lack of effect of glucosamine sulphate on aggrecan mRNA expression and (35)S-sulphate incorporation in bovine primary chondrocytes

Biochimica Et Biophysica Acta
Cheng-Juan QuMikko J Lammi

Abstract

Glucosamine and glucosamine sulphate have been promoted as a disease-modifying agent to improve the clinical symptoms of osteoarthritis. The precise mechanism of the action of the suggested positive effect of glucosamine or glucosamine sulphate on cartilage proteoglycans is not known, since the level of glucosamine in plasma remains very low after oral administration of glucosamine sulphate. We examined whether exogenous hexosamines or their sulphated forms would increase steady-state levels of aggrecan and hyaluronan synthase (HAS) or glycosaminoglycan synthesis using Northern blot and (35)S-sulphate incorporation analyses. Total RNA was extracted from bovine primary chondrocytes which were cultured either in 1 mM concentration of glucosamine, galactosamine, mannosamine, glucosamine 3-sulphate, glucosamine 6-sulphate or galactosamine 6-sulphate for 0, 4, 8 and 24 h, or in three different concentrations (control, 100 microM and 1 mM) of glucosamine sulphate salt or glucose for 24 or 72 h. Northern blot assay showed that neither hexosamines nor glucosamine sulphate salt stimulated aggrecan and HAS-2 mRNA expression. Glycosaminoglycan synthesis remained at a control level in the treated cultures, with the exception of mannosamine...Continue Reading

References

Nov 10, 1978·Calcified Tissue Research·E J ThonarG Lyons
Jan 1, 1992·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·T AignerK von der Mark
Nov 15, 1992·Analytical Biochemistry·J Rao, W R Otto
Dec 1, 1989·Analytical Biochemistry·G G NemethM E Bolander
Apr 1, 1988·Arthritis and Rheumatism·D H Manicourt, J C Pita
Jan 1, 1997·The Biochemical Journal·R B Lee, J P Urban
Feb 29, 2000·The Western Journal of Medicine·J P RindoneG Arriola
Jul 25, 2000·Osteoarthritis and Cartilage·J I FentonM W Orth
Nov 9, 2000·Seminars in Arthritis and Rheumatism·R D Altman
Mar 1, 1994·Osteoarthritis and Cartilage·H Müller-FassbenderI Setnikar
Mar 21, 2002·British Journal of Community Nursing·Rachael Ruane, Peter Griffiths
Aug 29, 2003·The Biochemical Journal·Paula J Mroz, Jeremiah E Silbert
Nov 8, 2003·Rheumatic Diseases Clinics of North America·Tim McAlindon
Jul 9, 2004·The Journal of Pharmacology and Experimental Therapeutics·Mark W PoustieSuzanne M Bernier
Oct 13, 2004·Arthritis and Rheumatism·Jolanda CibereJohn M Esdaile

❮ Previous
Next ❯

Citations

May 15, 2009·Cell and Tissue Research·Cheng-Juan QuMikko J Lammi
Mar 4, 2009·Glycobiology·Jeremiah E Silbert
Mar 17, 2007·Connective Tissue Research·Cheng-Juan QuJuha Töyräs
Jun 27, 2015·Journal of Agricultural and Food Chemistry·Yuliya HrynetsMirko Betti
Aug 12, 2014·Cell and Tissue Research·Janne H YlärinneMikko J Lammi
Sep 4, 2008·American Journal of Veterinary Research·Christopher R ByronHolly C Pondenis
Sep 4, 2008·American Journal of Veterinary Research·Christopher R ByronHolly C Pondenis
Jan 8, 2020·Drug Delivery and Translational Research·Helen ChattopadhyaySriparna Datta

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.