The lack of Pneumococcal surface protein C (PspC) increases the susceptibility of Streptococcus pneumoniae to the killing by microglia

Medical Microbiology and Immunology
Samuele PeppoloniElisabetta Blasi

Abstract

Microglial cells, the resident phagocytes in the brain, share many phenotypical and functional characteristics with peripheral macrophages, suggesting that they may participate in an innate immune response against microorganisms invading the central nervous system (CNS). In this study, we demonstrate that the microglial cells constitutively exhibit antibacterial activity in vitro against Streptococcus pneumoniae. By using a Pneumococcal surface protein C (PspC)-deleted strain and its wild-type counterpart, we found that the extent of such an activity is significantly influenced by the presence of a PspC molecule on the bacterial surface. The PspC- mutant FP20 is indeed more susceptible than the PspC+ strain HB565 to microglial killing. Interestingly, this phenomenon is observed when using a medium supplemented with heat-inactivated foetal bovine serum (FBS). Electron microscopy studies indicate that the microglial cells interact more efficiently with PspC- than with PspC+ pneumococci. Moreover, upon infection with the PspC- mutant, microglial cells produce levels of TNF-alpha, MIP-2, IL-10 and nitric oxide, significantly higher than those observed with PspC+ bacteria. These findings indicate that the lack of PspC significantly ...Continue Reading

References

Sep 4, 2009·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Monica M Mariani, Tammy Kielian
Sep 26, 2013·BMC Infectious Diseases·Susanna RicciGianni Pozzi
Oct 6, 2006·FEMS Microbiology Letters·Lisa R QuinLarry S McDaniel
Apr 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sven HammerschmidtPeter F Zipfel

Citations

Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·D T Fearon
May 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M K Pangburn, H J Müller-Eberhard
Apr 1, 1992·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D M Musher
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Seppo Meri, M K Pangburn
May 11, 1991·Reviews of Infectious Diseases·R B Johnston
May 1, 1990·Journal of Neuroimmunology·Elisabetta BlasiF Bistoni
Jul 1, 1988·Reviews of Infectious Diseases·David E BrilesLarry S McDaniel
Mar 1, 1995·Infection and Immunity·D R CundellElaine Tuomanen
Oct 1, 1996·Immunology Today·B P Morgan, P Gasque
Oct 25, 1996·Annals of the New York Academy of Sciences·Elaine Tuomanen
Jan 1, 1997·Annual Review of Immunology·U BoehmJ C Howard
Sep 18, 1997·The Journal of Infectious Diseases·I Idänpään-HeikkiläElaine Tuomanen
Jan 1, 1997·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Elaine Tuomanen, H R Masure
Jan 1, 1997·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·David E BrilesM J Crain
May 16, 1998·Trends in Microbiology·James C Paton
Jul 17, 1998·The Journal of Clinical Investigation·A RingElaine Tuomanen
Apr 15, 1999·Annual Review of Neuroscience·F González-Scarano, G Baltuch
Apr 30, 1999·Progress in Neurobiology·W J StreitN A Pennell
Sep 24, 1999·The Pediatric Infectious Disease Journal·H R ShinefieldJ Hansen
Sep 1, 2000·The Journal of Biological Chemistry·Robert JanulczykLars Björck
Dec 13, 2000·The Journal of Biological Chemistry·Jens HellwageSeppo Meri
Oct 27, 2001·The Journal of Infectious Diseases·Johann S BraunJ L Cleveland
Feb 2, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hanna JarvaSeppo Meri
Jun 18, 2002·Research in Microbiology·Barbara J PearceGianni Pozzi
Jul 18, 2002·Scandinavian Journal of Immunology·T Wuorimaa, H Käyhty
Apr 23, 2004·Infection and Immunity·Francesco IannelliGianni Pozzi
Jul 9, 2004·The Journal of Experimental Medicine·Janine ZweignerElaine Tuomanen
Oct 13, 2004·The Journal of Infectious Diseases·Carlos J OrihuelaElaine Tuomanen
Nov 6, 2004·The Journal of Infectious Diseases·Lauren MitchellElaine Tuomanen

Related Concepts

Microorganism
Immune Response
Mycoplasma pneumoniae
Bacterial Proteins
Tumor Necrosis Factor-alpha
Virus Viability
Macrophage inflammatory protein 2
Protein C, human
Recombinant Interleukin-10
Brain

Related Feeds

Bacterial Pneumonia (ASM)

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.

Bacterial Pneumonia

Bacterial pneumonia is a prevalent and costly infection that is a significant cause of morbidity and mortality in patients of all ages. Here is the latest research.