The Lactobacillus Bile Salt Hydrolase Repertoire Reveals Niche-Specific Adaptation

MSphere
Sarah O'FlahertyRodolphe Barrangou

Abstract

Various Lactobacillus species have been reported to deconjugate bile acids in the gastrointestinal tract (GIT) through the action of bile salt hydrolase (BSH) proteins. This function contributes to altering the gut microbiota composition and bile metabolism and detoxification and to lowering cholesterol levels. Here, we investigated the Lactobacillus BSH repertoire across 170 sequenced species. We used hidden Markov models to distinguish between BSH and closely related penicillin-V acylase (PVA) proteins. Even though BSH and PVA proteins have very different target substrates, they share high sequence similarity and are often misannotated. We determined that 82/170 (48.24%) species encoded PVA proteins, 39/170 (22.94%) species encoded BSH proteins, and 8/170 (4.71%) species encoded both BSH and PVA proteins, while 57/170 (33.53%) species encoded neither. Mapping the occurrence of BSH-encoding species onto a phylogenetic tree revealed that BSH-encoding lactobacilli primarily adopt the vertebrate-adapted lifestyle but not the environmental or plant-associated subsets. Phylogenetic analysis of the BSH sequences revealed two distinct clades, several conserved motifs, and the presence of six previously reported active-site residues. ...Continue Reading

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Oct 10, 2019·Gut Microbes·Jenessa A Winston, Casey M Theriot
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Datasets Mentioned

BETA
SRR6329247
SRR6329248
33323
and
SRR6763418

Methods Mentioned

BETA
RNA-seq
electrophoresis
Genotyping
PCR

Software Mentioned

CLC Genomics workbench
CLC Genomics (
Fastq
HMMER
HMM
Geneious
CLC Genomics
bcl2fastq
Geneious Biomatters
BLASTX

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