The Lactobacillus casei ptsHI47T mutation causes overexpression of a LevR-regulated but RpoN-independent operon encoding a mannose class phosphotransferase system

Journal of Bacteriology
Alain MazéAxel Hartke

Abstract

A proteome analysis of Lactobacillus casei mutants that are affected in carbon catabolite repression revealed that a 15-kDa protein was strongly overproduced in a ptsHI47T mutant. This protein was identified as EIIA of a mannose class phosphotransferase system (PTS). A 7.1-kb DNA fragment containing the EIIA-encoding open reading frame and five other genes was sequenced. The first gene encodes a protein resembling the RpoN (sigma54)-dependent Bacillus subtilis transcription activator LevR. The following pentacistronic operon is oriented in the opposite direction and encodes four proteins with strong similarity to the proteins of the B. subtilis Lev-PTS and one protein of unknown function. The genes present on the 7.1-kb DNA fragment were therefore called levR and levABCDX. The levABCDX operon was induced by fructose and mannose. No "-12, -24" promoter typical of RpoN-dependent genes precedes the L. casei lev operon, and its expression was therefore RpoN independent but required LevR. Phosphorylation of LevR by P approximately His-HPr stimulates its activity, while phosphorylation by P approximately EIIBLev inhibits it. Disruption of the EIIBLev-encoding levB gene therefore led to strong constitutive expression of the lev operon...Continue Reading

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Citations

Jul 24, 2007·Applied and Environmental Microbiology·Yong Jun GohRobert W Hutkins
Oct 10, 2006·Applied and Environmental Microbiology·Yong Jun GohRobert W Hutkins
Jan 17, 2012·Applied and Environmental Microbiology·Pablo MorteraChristian Magni
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Dec 22, 2006·Journal of Molecular Microbiology and Biotechnology·Vicente MonederoJosef Deutscher
Aug 19, 2007·Journal of Molecular Microbiology and Biotechnology·Sophie BeaufilsAxel Hartke
Jul 4, 2019·Journal of Molecular Microbiology and Biotechnology·Thanh Nguyen CaoJosef Deutscher

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