The landscape of gene mutations and clinical significance of tumor mutation burden in patients with soft tissue sarcoma who underwent surgical resection and received conventional adjuvant therapy.

The International Journal of Biological Markers
Li-Bin XuSheng-Ji Yu

Abstract

The aim of this study was to evaluate the landscape of gene mutations and the clinical significance of tumor mutation burden (TMB) in patients with soft tissue sarcoma who underwent surgical resection and received conventional adjuvant therapy. A total of 68 patients with soft tissue sarcoma were included. Postoperative tumor tissue specimens from the patients were collected for DNA extraction. Targeted next-generation sequencing of cancer-relevant genes was performed for the detection of gene mutations and the analysis of TMB. Univariate analysis between TMB status and prognosis was carried out using the Kaplan-Meier survival analysis, and multivariate analysis was adjusted by the Cox regression model. No specific genetic mutations associated with soft tissue sarcoma were found. The mutation frequency of TP53, PIK3C2G, NCOR1, and KRAS of the 68 patients with soft tissue sarcoma were observed in 19 cases (27.94%), 15 cases (22.06%), 14 cases (20.59%), and 14 cases (20.59%), respectively. With regard to the analysis of TMB, the overall TMB of the 68 patients with soft tissue sarcoma was relatively low (median: 2.05 per Mb (range: 0∼15.5 per Mb)). Subsequently, TMB status was divided into TMB-Low and TMB-Middle according to the m...Continue Reading

References

Apr 21, 1999·Current Opinion in Cell Biology·S J LeeversM D Waterfield
Jul 15, 2011·Nature Reviews. Cancer·Barry S TaylorMarc Ladanyi
Nov 23, 2013·PloS One·Nicolai Juul BirkbakZhigang C Wang
Dec 1, 2015·The International Journal of Biological Markers·Jung-Soo PyoJin Hee Sohn
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Aug 9, 2016·The Lancet Oncology·Mandy L BallingerUNKNOWN International Sarcoma Kindred Study
Aug 16, 2016·Surgical Pathology Clinics·Adrián Mariño-Enríquez, Judith V M G Bovée
Jan 18, 2017·Experimental and Molecular Pathology·Paul J LeeEric J Duncavage
Nov 4, 2017·Cell·UNKNOWN Cancer Genome Atlas Research Network. Electronic address: elizabeth.demicco@sinaihealthsystem.ca, UNKNOWN Cancer Genome Atlas Research Network
Dec 21, 2017·The New England Journal of Medicine·Mark YarchoanElizabeth M Jaffee
Apr 15, 2018·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Yuki Owada-OzakiHiroyuki Suzuki
May 13, 2018·Journal of the National Comprehensive Cancer Network : JNCCN·Margaret von MehrenJillian L Scavone
Jul 27, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael OffinMatthew D Hellmann
Aug 15, 2018·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Siddhartha DevarakondaStefan Michiels
Dec 7, 2018·Translational Lung Cancer Research·Laurent GreillierFabrice Barlesi
Dec 7, 2018·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Xiaolong YuJing Hu
Jan 16, 2019·Nature Genetics·Robert M SamsteinLuc G T Morris
Jan 22, 2019·The Journal of Veterinary Medical Science·Hajime AsadaHajime Tsujimoto
Feb 4, 2019·Anticancer Research·Michail SiderisFredric Willmott
May 16, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Wenfeng FangLi Zhang
May 28, 2019·Current Treatment Options in Oncology·Makoto EndoYoshihiro Matsumoto
Jun 4, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Katarzyna GuzikTad A Holak
Jun 7, 2019·World Journal of Gastroenterology : WJG·Changhoon ChoiHee Chul Park
Jun 7, 2019·Current Treatment Options in Oncology·Laurent MhannaJulien Mazieres

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Methods Mentioned

BETA
surgical resection
exome sequencing
PCR
DNA assay

Software Mentioned

Stata
SPSS
Integrative Genomics Viewer
BWA aligner
GATK

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