PMID: 7535922Mar 11, 1995Paper

The large subunit of HIV-1 reverse transcriptase interacts with beta-actin

Nucleic Acids Research
M HottigerU Hübscher

Abstract

HIV-1 reverse transcriptase is a dimeric enzyme mainly involved in the replication of the viral genome. A filamentous phage cDNA expression library from human lymphocytes was used to select cellular proteins interacting with HIV-1 reverse transcriptase Affinity selections using the bacterially expressed monomeric large subunit of reverse transcriptase (p66) yielded host beta-actin. This clone was expressed as glutathione-S-transferase fusion protein which was identified by using a specific antibody against beta-actin. Furthermore we show that also the eukaryotic beta-actin binds to either the large subunit of reverse transcriptase or to the Pol precursor polyprotein in vitro. The reverse transcriptase/beta-actin interaction might be important for the secretion of HIV-1 virions.

References

Nov 1, 1977·The Journal of Cell Biology·C H DamskyL Warren
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P HafkemeyerU Hübscher
Jul 2, 1991·Biochemistry·A Jacobo-Molina, E Arnold
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M Bryant, L Ratner
Jan 1, 1986·Journal of Cell Science. Supplement·R A Mortara, G L Koch
Mar 1, 1985·European Journal of Biochemistry·H Strzelecka-GołaszewskaM Mossakowska
Feb 1, 1971·European Journal of Biochemistry·U Lindberg, S Eriksson
Jan 30, 1984·Biochemical and Biophysical Research Communications·L StanislawskyV Moura Neto
Jan 21, 1994·Journal of Molecular Biology·A GragerovM E Gottesman

❮ Previous
Next ❯

Citations

Oct 15, 2013·Trends in Cell Biology·Raphaël GaudinPhilippe Benaroch
Feb 14, 2004·Methods : a Companion to Methods in Enzymology·Claudio RhynerReto Crameri
Oct 1, 1996·Current Opinion in Biotechnology·I S Dunn
Dec 16, 1998·Immunotechnology : an International Journal of Immunological Engineering·P GrobM Suter
Feb 16, 2011·The Journal of Biological Chemistry·Paul J VorsterYuntao Wu
Jul 1, 1996·The Journal of Experimental Medicine·R CrameriK Blaser
Nov 16, 2001·AIDS Research and Human Retroviruses·F J IbarrondoP Krogstad
May 30, 2012·Retrovirology·Mark SpearYuntao Wu
Oct 19, 2013·Viruses·Jonas KüglerMichael Hust
Jan 1, 2007·Future Virology·Sergey Iordanskiy, Michael Bukrinsky
Sep 13, 2008·Journal of Molecular Biology·Julie LemayLang Xia Liu
Sep 15, 2009·Reviews in Medical Virology·David WarrilowDavid Harrich
Oct 13, 2012·Communicative & Integrative Biology·Xuehua XuYuntao Wu
May 1, 2020·Journal of Virology·Anastassia MikhailovaAsier Sáez-Cirión
Feb 1, 1996·Biological Chemistry Hoppe-Seyler·Y M HenskensA V Nieuw Amerongen
Sep 1, 1996·Journal of Virology·M E PerottiD M Phillips
Aug 19, 2008·Journal of Proteome Research·Adam BurgenerFrancis Plummer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.