The last-born daughter cell contributes to division orientation of Drosophila larval neuroblasts

Nature Communications
Nicolas Loyer, Jens Januschke

Abstract

Controlling the orientation of cell division is important in the context of cell fate choices and tissue morphogenesis. However, the mechanisms providing the required positional information remain incompletely understood. Here we use stem cells of the Drosophila larval brain that stably maintain their axis of polarity and division between cell cycles to identify cues that orient cell division. Using live cell imaging of cultured brains, laser ablation and genetics, we reveal that division axis maintenance relies on their last-born daughter cell. We propose that, in addition to known intrinsic cues, stem cells in the developing fly brain are polarized by an extrinsic signal. We further find that division axis maintenance allows neuroblasts to maximize their contact area with glial cells known to provide protective and proliferative signals to neuroblasts.

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Citations

Aug 25, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Alicia G GubiedaJosana Rodriguez
Sep 30, 2020·Molecular Biology of the Cell·Elias T Spiliotis, Michael A McMurray
Dec 7, 2020·Current Opinion in Structural Biology·Cayetano Gonzalez
May 26, 2021·Current Biology : CB·Elias T Spiliotis, Konstantinos Nakos
Aug 10, 2020·Current Opinion in Cell Biology·Melissa K Delgado, Clemens Cabernard
May 18, 2021·The EMBO Journal·Martim Dias Gomes, Sandra Iden

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Methods Mentioned

BETA
environmental stresses
dissection
fluorescence recovery after photobleaching

Software Mentioned

Zen
Ilastik
ImageJ BoneJ

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