The Last Breath: A μCT-based method for investigating the tracheal system in Hexapoda

Arthropod Structure & Development
Dariusz IwanMarcin Raś

Abstract

In recent years, μCT-based studies of the insect tracheal system have become an increasingly important area of research. Nevertheless, the methods proposed in previous research for investigating the respiratory system in the three-dimensional space were described and tested based on a relatively small number of specimens. Additionally, the individuals studied in all these cases represented only a single post-embryonic stadium - pupa or imago - of a particular insect species. Therefore, in the current situation it is difficult to predict the reliability and possible limitations of these methods. To address this problem we conducted a methodological study, during which we used 65 individuals representing larvae, pupae and imagines of the mealworm beetle (Tenebrio molitor). In addition to the protocol previously described, which implicated freezing as a killing technique, we also tested a novel one, which was based on ethyl acetate fumigation of the specimens studied. We included step-by-step guides for the manual and semiautomatic approaches in order to facilitate the digital visualization of the tracheal system. Our investigations enabled us to generate multiple models of the tracheal system of all post-embryonic stages of the m...Continue Reading

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Citations

Oct 12, 2016·Scientific Reports·Ryuta MizutaniKentaro Uesugi
Aug 9, 2016·Arthropod Structure & Development·Benjamin AberleJan-Henning Dirks
Nov 22, 2019·Scientific Reports·Ignacio Alba-AlejandreFernando E Vega
Feb 8, 2021·Journal of Insect Physiology·Philipp LehmannJohn S Terblanche
Nov 28, 2020·Arthropod Structure & Development·Gero HilkenAndy Sombke
Oct 8, 2021·Nature Communications·Long-Hai WangMinglin Ma

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