The latency of peroxisomal catalase in terms of effectiveness factor for pancreatic and glioblastoma cancer cell lines in the presence of high concentrations of H2O2: Implications for the use of pharmacological ascorbate in cancer therapy.

Free Radical Biology & Medicine
Dieanira ErudaitiusVictor G J Rodgers

Abstract

Previous research has identified variation in cancer cell line response to high levels of extracellular H2O2 (eH2O2) exposure. This directly contributes to our understanding cellular efficacy of pharmacological ascorbate (P-AscH-) therapy. Here we investigate the factors contributing to latency of peroxisomal catalase of a cell and the importance of latency in evaluating cell exposure to eH2O2. First, we develop a mathematical framework for the latency of catalase in terms of an effectiveness factor, ηeff, to describe the catalase activity in the presence of high levels of eH2O2. A simplified relationship emerges, [Formula: see text] when mprp/Dij≪1, where mp,rp, and [Formula: see text] are the experimentally determined peroxisome permeability, average peroxisome radius, and the pseudo first-order reaction rate constant, respectively. [Formula: see text] is the catalase concentration in the peroxisome and k2=1.7x107M-1s-1. Next, previously published parameters are used to determine the latency effect of the cell lines: normal pancreatic cells (H6c7), pancreatic cancer cells (MIA PaCa-2), and glioblastoma cells (LN-229, T98G, and U-87), all which vary in their susceptibility to exposure to high eH2O2. The results show that effec...Continue Reading

References

Apr 1, 1966·Physiological Reviews·C De Duve, P Baudhuin
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H N Kirkman, G F Gaetani
Jun 20, 2000·FEBS Letters·F Antunes, E Cadenas
Sep 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·Qi ChenMark Levine
Dec 13, 2006·Trends in Biochemical Sciences·Henry N Kirkman, Gian F Gaetani
May 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Qi ChenMark Levine
Aug 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qi ChenMark Levine
Jan 14, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Juan DuJoseph J Cullen
Mar 7, 2012·Molecular Carcinogenesis·Juan DuJoseph J Cullen
Dec 5, 2012·Free Radical Research·K E OlneyJ J Cullen
Jun 15, 2013·Cancer Research·Malvika RawalJoseph J Cullen
Jul 9, 2013·Methods in Enzymology·H Susana MarinhoFernando Antunes
Feb 11, 2014·Free Radical Biology & Medicine·Zita A SibenallerJoseph J Cullen
Feb 6, 2018·Clinical & Experimental Metastasis·Justin G WilkesJoseph J Cullen
Dec 24, 2018·Seminars in Radiation Oncology·Joshua D SchoenfeldJoseph J Cullen
Jul 25, 2019·Molecular Cancer Research : MCR·Visarut BuranasudjaGarry R Buettner
Aug 21, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Bryan G AllenJohn M Buatti
Dec 4, 2019·Frontiers in Veterinary Science·Margaret L MusserChad M Johannes
Feb 6, 2020·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·Ewa Lucja GregoraszczukAleksandra Such

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Citations

Jun 3, 2021·International Journal of Molecular Sciences·Charlotta Bengtson, Annemie Bogaerts

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