PMID: 3745270Sep 1, 1986Paper

The lateral diffusion of lipid probes in the surface membrane of Schistosoma mansoni

The Journal of Cell Biology
M FoleyI Moore

Abstract

The technique of fluorescence recovery after photobleaching was used to measure the lateral diffusion of fluorescent lipid analogues in the surface membrane of Schistosoma mansoni. Our data reveal that although some lipids could diffuse freely others exhibited restricted lateral diffusion. Quenching of lipid fluorescence by a non-permeant quencher, trypan blue, showed that there was an asymmetric distribution of lipids across the double bilayer of mature parasites. Those lipids that diffused freely were found to reside mainly in the external monolayer of the outer membrane whereas lipids with restricted lateral diffusion were located mainly in one or more of the monolayers beneath the external monolayer. Formation of surface membrane blebs allowed us to measure the lateral diffusion of lipids in the membrane without the influence of underlying cytoskeletal structures. The restricted diffusion found on the normal surface membrane of mature parasites was found to be released in membrane blebs. Quenching of fluorescent lipids on blebs indicated that all probes were present almost entirely in the external monolayer. Juvenile worms exhibited lower lateral diffusion coefficients than mature parasites: in addition, the lipids partitio...Continue Reading

References

Sep 8, 1977·Nature·D J Mclaren, D J Hockley
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J W ParceH M McConnell
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P DragstenJ Schlessinger
Jan 1, 1978·Annual Review of Biochemistry·L Stryer
Dec 20, 1979·Biochimica Et Biophysica Acta·R J Cherry
Nov 1, 1976·Biophysical Journal·D E KoppelW W Webb
Jun 12, 1985·Biochimica Et Biophysica Acta·E Blatt, W H Sawyer
Feb 18, 1972·Science·S J Singer, G L Nicolson
Mar 1, 1972·International Journal for Parasitology·J A Clegg, S R Smithers
Jan 1, 1973·International Journal for Parasitology·D J Hockley, D J McLaren
Feb 1, 1974·Experimental Parasitology·D G Colley, S K Wikel
Jan 1, 1967·Transactions of the Royal Society of Tropical Medicine and Hygiene·S R Smithers, R J Terry
Jan 1, 1971·Transactions of the Royal Society of Tropical Medicine and Hygiene·D Hockley, D McLaren
Mar 27, 1980·Biochimica Et Biophysica Acta·N L Thompson, D Axelrod
May 17, 1982·FEBS Letters·K UtsumiM Miyahara
Jun 1, 1982·Biophysical Journal·B M Aizenbud, N D Gershon
Aug 1, 1982·The Journal of Cell Biology·J C SamuelsonJ R David
Oct 30, 1981·Biochemical and Biophysical Research Communications·N SahyounP Cuatrecasas
Apr 1, 1965·Experimental Parasitology·J A CLEGG

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Citations

Jan 15, 1987·Molecular and Biochemical Parasitology·M W KennedyP B Garland
May 1, 1987·Molecular and Biochemical Parasitology·W Cushley, J R Kusel
Jan 1, 1991·Parasitology Today·S T Furlong
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·L Q Vieira, J R Kusel
Dec 11, 1999·Molecular and Biochemical Parasitology·E L RacoosinE J Pearce
Aug 1, 1992·Parasitology·D Moffat, J R Kusel
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J M el Hage ChahineP F Devaux
Dec 11, 2008·Photochemistry and Photobiology·Wor Thongthai, Keith Weninger
Feb 20, 2007·The Journal of Parasitology·John H KeatingPatrick J Skelly
Aug 15, 2013·Cell Biochemistry and Biophysics·Federica MigliardoRashika El Ridi
Jan 27, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Mark J RichardsSusan Daniel
Oct 19, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Arshiya F MohammedJean Gariépy
Apr 29, 2011·Molecular and Biochemical Parasitology·Hatem TallimaRashika El Ridi
Mar 28, 2006·Cytokine·Rashika El RidiHatem Tallima
Apr 12, 2016·Biochimica Et Biophysica Acta. General Subjects·Rashika El RidiFederica Migliardo
Feb 25, 2010·Current Biology : CB·Manfred Kayser
Oct 23, 1989·FEBS Letters·J F TocanneJ F Tournier
Jan 18, 2003·American Journal of Human Genetics·Manfred KayserMark Stoneking
Mar 1, 1994·FEMS Immunology and Medical Microbiology·D HulseP C Wilkinson
Jan 13, 2010·Molecular Pharmaceutics·Silvia PetersenHeike Bunjes

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