The Ld Cht1 gene encodes the secretory chitinase of the human pathogen Leishmania donovani

Gene
Alison M Shakarian, D M Dwyer

Abstract

Leishmania donovani promastigotes were shown to release chitinase activity during their growth in vitro. A PCR-based strategy identified a single copy ORF capable of encoding the L. donovani chitinase (Ld Cht1, 1374 bp). Ld Cht1 was shown to be actively transcribed by L. donovani promastigotes using reverse transcription and PCR amplification. The deduced aa sequence of Ld Cht1 showed high conservation to known chitinases including the putative active and two substrate binding sites. Antiserum generated against four peptides derived from its deduced aa sequence immunoprecipitated an approx. 50-kDa in vitro transcription/translation product of Ld Cht1. Further, this antiserum also immunoprecipitated both the native L. donovani 50-kDa Cht1 protein and the native chitinase activity synthesized and released by these parasites. Cumulatively, these data demonstrated that Ld Cht1 encoded the chitinase of this important human pathogen.

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Citations

May 28, 2009·Cell and Tissue Research·Jovana Sádlová, Petr Volf
Mar 30, 2010·Molecular and Cellular Biochemistry·Alison M ShakarianDennis M Dwyer
Oct 18, 2003·Insect Biochemistry and Molecular Biology·Zhengrong HaoSerap Aksoy
Mar 24, 2004·International Journal for Parasitology·Alain DebrabantDennis M Dwyer
Feb 19, 2002·International Journal for Parasitology·William F SopwithPaul A Bates
Nov 14, 2002·Insect Biochemistry and Molecular Biology·Zhengrong Hao, Serap Aksoy
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May 3, 2018·Expert Opinion on Therapeutic Targets·Shyam Sundar, Bhawana Singh
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Feb 14, 2020·Pathogens and Disease·Sajad RashidiGholamreza Hatam
Nov 13, 2002·The Journal of Biological Chemistry·Alejandro PadillaAlain Debrabant
Jun 6, 2006·Molecular and Cellular Biochemistry·Diane E MarottaDennis M Dwyer

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