The Leaderless Bacteriocin Enterocin K1 Is Highly Potent against Enterococcus faecium: A Study on Structure, Target Spectrum and Receptor

Frontiers in Microbiology
Kirill V OvchinnikovDzung B Diep

Abstract

Enterocin K1 (EntK1), enterocin EJ97 (EntEJ97), and LsbB are three sequence related leaderless bacteriocins. Yet LsbB kills only lactococci while EntK1 and EntEJ97 target wider spectra with EntK1 being particularly active against Enterococcus faecium, including nosocomial multidrug resistant isolates. NMR study of EntK1 showed that it had a structure very similar to LsbB - both having an amphiphilic N-terminal α-helix and an unstructured C-terminus. The α-helix in EntK1 is, however, about 3-4 residues longer than that of LsbB. Enterococcal mutants highly resistant to EntEJ97 and EntK1 were found to have mutations within rseP, a gene encoding a stress response membrane-bound Zn-dependent protease. Heterologous expression of the enterococcal rseP rendered resistant cells of Streptococcus pneumoniae sensitive to EntK1 and EntEJ97, suggesting that RseP likely serves as the receptor for EntK1 and EntEJ97. It was also shown that the conserved proteolytic active site in E. faecalis RseP is partly required for EntK1 and EntEJ97 activity, since alanine substitutions of its conserved residues (HExxH) reduced the sensitivity of the clones to the bacteriocins. RseP is known to be involved in bacterial stress response. As expected, the grow...Continue Reading

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Citations

Feb 16, 2019·Current Protein & Peptide Science·Marian VincenziMarilisa Leone
Aug 8, 2018·FEMS Microbiology Reviews·Jeella Z AcedoMarco J van Belkum
Sep 21, 2018·Frontiers in Microbiology·Rodney H PerezKenji Sonomoto
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Oct 30, 2020·International Journal of Molecular Sciences·Aleksandra TymoszewskaTamara Aleksandrzak-Piekarczyk
Jul 8, 2021·Scientific Reports·Christian KranjecDzung B Diep

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Methods Mentioned

BETA
NMR
PCR
environmental stress

Software Mentioned

PONDR
TALOS
VL
MolMol
Topspin
CYANA
CARA
Clustal Omega

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