PMID: 7514154May 15, 1994Paper

The lectin Griffonia simplicifolia I-A4 (GS I-A4) specifically recognizes terminal alpha-linked N-acetylgalactosaminyl groups and is cytotoxic to the human colon cancer cell lines LS174t and SW1116

International Journal of Cancer. Journal International Du Cancer
Y F ChenI J Goldstein

Abstract

The lectin GS I-A4 binds to terminal alpha-N-acetylgalactosaminyl (GalNAc) groups (which include the Tn antigen), but not to the closely related tumor-associated epitope, sialylated Tn antigen. The lectin also precipitates asialo OSM, but not its native sialylated form. Lectin histochemistry with human colonic tissues showed that GS I-A4 specifically stained specimens of colon cancer and colonic tissues from individuals with FAP; however, normal colonic tissues from patients without colonic disease were rarely stained with this lectin. Glycoconjugates bound by GS I-A4 were observed on the surface membranes of 2 human colon cancer cell lines, LS174t and SW1116, when fluorescein isothiocyanate (FITC)-conjugated GS I-A4 was used. GS I-A4 was toxic to these 2 human colon cancer cell lines in monolayer culture. A dose-response study conducted using 10-160 micrograms/ml, of GS I-A4 demonstrated significant dose-related toxicity against LS174t and SW1116 cells. At concentrations > 80 micrograms/ml, > 99% of LS174t and > 90% of SW1116 cells were killed. Four mM GalNAc specifically inhibited the cytotoxic effect of GS I-A4 (p < 0.001), whereas 4mM N-acetylglucosamine (GlcNAc) had no effect. Two other lectins that recognize terminal alph...Continue Reading

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Citations

May 31, 2012·Journal of Natural Medicines·Andrea MedeirosEduardo Osinaga
Mar 23, 1999·The American Journal of Gastroenterology·D J DesiletsR K Wong
Jun 10, 2017·International Journal of Molecular Sciences·Guillaume PoirouxPierre Rougé
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Behjatolah Monzavi-KarbassiThomas Kieber-Emmons
Oct 9, 2019·International Journal of Biological Macromolecules·Ajay Kumar GautamKhem Chand Saini
Jun 24, 2020·International Journal of Biological Macromolecules·Benildo Sousa CavadaKyria Santiago Nascimento
Aug 15, 2021·Glycoconjugate Journal·Fulvio ReggioriEeva-Liisa Eskelinen

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