The Legionella effector protein DrrA AMPylates the membrane traffic regulator Rab1b

Science
Matthias P MüllerAymelt Itzen

Abstract

In the course of Legionnaires' disease, the bacterium Legionella pneumophila affects the intracellular vesicular trafficking of infected eukaryotic cells by recruiting the small guanosine triphosphatase (GTPase) Rab1 to the cytosolic face of the Legionella-containing vacuole. In order to accomplish this, the Legionella protein DrrA contains a specific guanine nucleotide exchange activity for Rab1 activation that exchanges guanosine triphosphate (GTP) for guanosine diphosphate on Rab1. We found that the amino-terminal domain of DrrA possesses adenosine monophosphorylation (AMPylation) activity toward the switch II region of Rab1b, leading to posttranslational covalent modification of tyrosine 77. AMPylation of switch II by DrrA restricts the access of GTPase activating proteins, thereby rendering Rab1b constitutively active.

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Citations

Apr 12, 2013·Immunologic Research·Amy D ProalInge A Lindseth
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Yan LiHaydn L Ball
Sep 29, 2011·Journal of the American Chemical Society·Markus GrammelHoward C Hang
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Jul 8, 2011·Nature·Yunhao Tan, Zhao-Qing Luo
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Aug 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Tamara J O'ConnorRalph R Isberg
Oct 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Stefan SchoebelAymelt Itzen
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yunhao TanZhao-Qing Luo
Mar 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lena K OesterlinAymelt Itzen
Mar 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Olena Pylypenko, Bruno Goud
Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Konstantin GavriljukKlaus Gerwert
Jul 31, 2013·Proceedings of the National Academy of Sciences of the United States of America·Konstantin GavriljukCarsten Kötting
Aug 9, 2012·The Journal of Biological Chemistry·Matthias P MüllerAymelt Itzen
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