The lens internal microcirculation system delivers solutes to the lens core faster than would be predicted by passive diffusion

American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Ehsan Vaghefi, Paul J Donaldson

Abstract

It has been proposed that optical properties of the lens are actively maintained by an internal microcirculation system that utilizes ionic and fluid fluxes to deliver nutrients to deeper regions of the lens tissue via the extracellular space faster than would occur by passive diffusion alone. To test this hypothesis, we utilized a range of commercially available magnetic resonance imaging (MRI) reagents of varying molecular sizes that served as tracers of extracellular solute delivery. The penetration of these tracers into bovine lenses incubated in the absence and presence of solutions that inhibit the microcirculation was monitored in real time over a 4-h period using T1-weighted MRI. We found that only the smaller contrast agents were delivered to the core of the lens and that the rate of solute penetration was significantly faster than that calculated simple diffusion. Next, the lenses were first incubated in either high extracellular K+ to depolarize the lens potential or ouabain to inhibit the Na+ pump. These two perturbations are known to inhibit the circulating ionic and fluid fluxes that are proposed to drive solute delivery into the lens core. Both perturbations inhibited the delivery of the extracellular tracer mole...Continue Reading

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Citations

May 30, 2019·Nutrients·Andrea J BraakhuisPaul J Donaldson
May 1, 2021·International Journal of Molecular Sciences·Sander De BruyneJoris R Delanghe
Sep 24, 2021·Investigative Ophthalmology & Visual Science·Zhen WangKevin L Schey
Dec 22, 2021·Expert Opinion on Therapeutic Targets·Barbara K Pierscionek

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

EZYFIT
MATLAB

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