The lens-regenerating competence in the outer cornea and epidermis of larval Xenopus laevis is related to pax6 expression.

Journal of Anatomy
Cesare GargioliS Cannata

Abstract

After lentectomy, larval Xenopus laevis can regenerate a new lens by transdifferentiation of the outer cornea and pericorneal epidermis (lentogenic area). This process is promoted by retinal factor(s) accumulated into the vitreous chamber. To understand the molecular basis of the lens-regenerating competence (i.e. the capacity to respond to the retinal factor forming a new lens) in the outer cornea and epidermis, we analysed the expression of otx2, pax6, sox3, pitx3, prox1, betaB1-cry (genes all involved in lens development) by Real-time RT-PCR in the cornea and epidermis fragments dissected from donor larvae. The same fragments were also implanted into the vitreous chamber of host larvae to ascertain their lens-regenerating competence using specific anti-lens antibodies. The results demonstrate that there is a tight correlation between lens-regenerating competence and pax6 expression. In fact, (1) pax6 is the only one of the aforesaid genes to be expressed in the lentogenic area; (2) pax6 expression is absent in head epidermis outside the lentogenic area and in flank epidermis, both incapable of transdifferentiating into lens after implantation into the vitreous chamber; (3) in larvae that have undergone eye transplantation un...Continue Reading

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Citations

Apr 25, 2012·Cell and Tissue Research·Sajjad Sisakhtnezhad, Maryam M Matin
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Apr 24, 2008·Journal of Anatomy·Stefano M CannataCesare Gargioli
Apr 15, 2019·Experimental Eye Research·Surabhi SonamJonathan J Henry

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