PMID: 9636182Jun 24, 1998Paper

The lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the Mediterranean fruit fly

Proceedings of the National Academy of Sciences of the United States of America
Alfred M HandlerS H Saul

Abstract

The piggyBac (IFP2) short inverted terminal repeat transposable element from the cabbage looper Trichoplusia ni was tested for gene transfer vector function as part of a bipartite vector-helper system in the Mediterranean fruit fly Ceratitis capitata. A piggyBac vector marked with the medfly white gene was tested with a normally regulated piggyBac transposase helper at two different concentrations in a white eye host strain. Both experiments yielded transformants at an approximate frequency of 3-5%, with a total of six lines isolated having pigmented eyes with various levels of coloration. G1 transformant siblings from each line shared at least one common integration, with several sublines having an additional second integration. For the first transformant line isolated, two integrations were determined to be stable for 15 generations. For five of the lines, a piggyBac-mediated transposition was verified by sequencing the insertion site junctions isolated by inverse PCR that identified a characteristic piggyBac TTAA target site duplication. The efficient and stable transformation of the medfly with a lepidopteran vector represents transposon function over a relatively large evolutionary distance and suggests that the piggyBac s...Continue Reading

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Citations

Apr 14, 2010·Transgenic Research·Holly J FergusonMalcolm Fraser
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Kathryn A RaphaelMarianne Frommer
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Ludvik M GomulskiAnna R Malacrida
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Katia KomitopoulouAnastassios C Mintzas
Feb 12, 2004·Insect Biochemistry and Molecular Biology·Alan S RobinsonPeter W Atkinson
Feb 13, 2001·Insect Biochemistry and Molecular Biology·A M Handler
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Alfred M Handler
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Carsten HornErnst A Wimmer
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Peter W Atkinson
Mar 26, 2003·Insect Biochemistry and Molecular Biology·M SumitaniM Hatakeyama
Aug 14, 2003·Insect Biochemistry and Molecular Biology·Raymond WilsonDavid A O'Brochta
Jun 8, 2002·Mutation Research·Alan S Robinson
Jan 15, 2000·Insect Molecular Biology·A M Handler, R A Harrell
Feb 15, 2000·Insect Molecular Biology·A C PinkertonP W Atkinson
Oct 13, 2000·Insect Molecular Biology·D A O'BrochtaM J Lehane
Jun 26, 2001·Insect Molecular Biology·K MichelP W Atkinson
Sep 17, 2002·Insect Molecular Biology·M D LorenzenR W Beeman
Oct 24, 2002·Development, Growth & Differentiation·Hongjie ZhangSumihare Noji
May 22, 2004·Bulletin of Entomological Research·M G Kramer
Jun 20, 2007·Nucleic Acids Research·Juan Cadiñanos, Allan Bradley
Mar 11, 1999·Genes & Development·M YamaoH Mori
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·B T BeerntsenB M Christensen
Sep 13, 2000·Genome Génome / Conseil National De Recherches Canada·M RosettoR Dallai

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