PMID: 9422614Jan 9, 1998Paper

The leuO gene product has a latent ability to relieve bgl silencing in Escherichia coli

Journal of Bacteriology
C UeguchiT Mizuno

Abstract

The Escherichia coli bgl operon is of interest, since its expression is silent (phenotypically Bgl-), at least under standard laboratory conditions. Here we attempted to identify a trans-acting factor(s) that is presumably relevant to the regulation of bgl by a random insertion mutagenesis with mini-Tn10. These collected mutations, conferring the phenotype of Bgl+, were localized in three loci on the genetic map, two of which appeared to be hns and bglJ, which were previously implicated as the factors affecting the Bgl phenotype. The other locus at 1 to 2 min on the genetic map appeared to be a new one. In this case, the insertion mutation was found to be just in front of the leuO gene encoding a putative LysR-like DNA-binding protein. Genetic analyses revealed that overproduction of LeuO in the wild-type cells causes the phenotype of Bgl+. A leuO deletion mutant was also characterized in terms of expression of bgl. From these results, the possible function of LeuO in bgl expression will be discussed from an evolutionary and/or ecological point of view.

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Citations

Apr 12, 2000·Molecular Microbiology·A Caramel, K Schnetz
May 6, 2004·Journal of Bacteriology·Marcos Fernández-MoraEdmundo Calva
Feb 16, 2005·The Journal of Biological Chemistry·Chien-Chung Chen, Hai-Young Wu
Jun 16, 2007·The Journal of Biological Chemistry·V NagarajavelKarin Schnetz
Oct 19, 2010·Journal of Bacteriology·G Raja VenkateshKarin Schnetz
Jul 21, 2012·Molecular Microbiology·Ismael Hernández-Lucas, Edmundo Calva
Jul 19, 2012·Molecular Microbiology·Shane C DillonCharles J Dorman
May 29, 2001·The Journal of Biological Chemistry·A MajumderH Y Wu
Jun 8, 2001·Journal of Bacteriology·F Repoila, S Gottesman
Mar 7, 2009·PLoS Genetics·T Sabari SankarKarin Schnetz
Dec 31, 2000·The Journal of Biological Chemistry·C C ChenH Y Wu
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Regine Hengge-Aronis
Oct 3, 2007·Molecular Microbiology·Miguel Angel De la CruzEdmundo Calva
Oct 17, 2009·PLoS Genetics·Zhongge Zhang, Milton H Saier
Oct 4, 2016·Frontiers in Cellular and Infection Microbiology·Hannes Breddermann, Karin Schnetz
Sep 28, 2000·Biochemical and Biophysical Research Communications·M FangH Y Wu
Jun 24, 2000·Research in Microbiology·E Tamburini, G Mastromei
Apr 4, 2000·Current Opinion in Microbiology·M Yarmolinsky
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Oct 1, 2021·Journal of Medical Microbiology·Marcos Fernández-MoraEdmundo Calva

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