PMID: 8592159Feb 1, 1996Paper

The Le(x) carbohydrate sequence is recognized by antibody to L5, a functional antigen in early neural development

Journal of Neurochemistry
A StreitC D Stern

Abstract

The L5 antigenic determinant was previously suggested to be a carbohydrate epitope present on murine cell recognition molecules in the developing brain and to be an early neural marker in the chick embryo. Here, we show that L5 immunoreactivity is associated with complex-type N-glycosidic oligosaccharides. To identify the carbohydrate structure recognized by the L5 antibody, we investigate its binding to N-linked oligosaccharides derived from L5 glycoproteins and to known glycans. Results of mass spectrometric analyses of L5-positive neoglycolipids prepared from L5 glycoproteins are consistent with those for N-glycans containing a 3-fucosyl N-acetyllactosamine sequence. We also investigate L5 binding to structurally defined, lipid-linked oligosaccharides based on the blood group type I and II backbones. Chromatogram binding assays, ELISA, and inhibition studies show that the antibody reacts strongly with carbohydrate chains presenting the 3-fucosyl N-acetyllactosamine sequence [Lewisx (Le(x)) or X-hapten] also recognized by anti-SSEA-1 and anti-CD15. Histochemical studies with different antibodies recognizing the Lex sequence show partially overlapping patterns of immunoreactivity during early neural development in the chick em...Continue Reading

Citations

Apr 20, 2001·Biological Chemistry·C AlbachB Schmitz
Oct 3, 2009·Cell and Tissue Research·Tim CzopkaAndreas Faissner
Aug 15, 2001·The Journal of Comparative Neurology·F ForutanC Andressen
May 20, 1998·Molecular Neurobiology·K C BreenF D Hayes
Aug 28, 2007·The Journal of Comparative Neurology·Alice KlausmeyerAndreas Faissner
May 12, 2009·Biological Chemistry·Yan LiuTen Feizi
Sep 28, 1998·The European Journal of Neuroscience·J K MaiK W Ashwell
Mar 30, 2001·The Journal of Biological Chemistry·Z CaoN Panjwani
Jan 27, 2007·Journal of Neurochemistry·Gabriele Loers, Melitta Schachner
Mar 29, 2014·The Journal of Biological Chemistry·David LutzMelitta Schachner
Dec 6, 2006·Glycobiology·Valentina BogoevskaChristoph Wagener
May 25, 2006·International Journal of Cancer. Journal International Du Cancer·Sabine RiethdorfKlaus Pantel
Mar 10, 2011·The Journal of Biological Chemistry·Eva HennenAndreas Faissner
Dec 2, 1999·Biochimica Et Biophysica Acta·T Endo
Jan 7, 2009·Analytical Biochemistry·Thomas IskratschIain B H Wilson
Jun 12, 2009·Carbohydrate Research·Gerard J A RouwendalDirk Bosch
Dec 8, 2009·European Journal of Cell Biology·Nainesh KatagihallimathMelitta Schachner

❮ Previous
Next ❯