The Lid/KDM5 histone demethylase complex activates a critical effector of the oocyte-to-zygote transition

PLoS Genetics
Daniela Torres-CampanaBenjamin Loppin

Abstract

Following fertilization of a mature oocyte, the formation of a diploid zygote involves a series of coordinated cellular events that ends with the first embryonic mitosis. In animals, this complex developmental transition is almost entirely controlled by maternal gene products. How such a crucial transcriptional program is established during oogenesis remains poorly understood. Here, we have performed an shRNA-based genetic screen in Drosophila to identify genes required to form a diploid zygote. We found that the Lid/KDM5 histone demethylase and its partner, the Sin3A-HDAC1 deacetylase complex, are necessary for sperm nuclear decompaction and karyogamy. Surprisingly, transcriptomic analyses revealed that these histone modifiers are required for the massive transcriptional activation of deadhead (dhd), which encodes a maternal thioredoxin involved in sperm chromatin remodeling. Unexpectedly, while lid knock-down tends to slightly favor the accumulation of its target, H3K4me3, on the genome, this mark was lost at the dhd locus. We propose that Lid/KDM5 and Sin3A cooperate to establish a local chromatin environment facilitating the unusually high expression of dhd, a key effector of the oocyte-to-zygote transition.

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Citations

Mar 7, 2020·PLoS Genetics·Paulo Navarro-Costa, Rui Gonçalo Martinho
Jul 22, 2020·G3 : Genes - Genomes - Genetics·Emir E Avilés-PagánTerry L Orr-Weaver
Oct 18, 2020·Current Opinion in Insect Science·Subba Reddy Palli

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Methods Mentioned

BETA
transgenic
RNA Seq
immunoprecipitation
ChIP
ChIP-Seq
PCR
Assay

Software Mentioned

picard
Photoshop
DESeq2
bcl2fastq
all
Integrative Genomics Viewer ( igv )
Deeptools
R
IGV
tools

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