The LIM Protein Ajuba Augments Tumor Metastasis in Colon Cancer

Cancers
Noëlle DommannDeborah Stroka

Abstract

Colorectal cancer, along with its high potential for recurrence and metastasis, is a major health burden. Uncovering proteins and pathways required for tumor cell growth is necessary for the development of novel targeted therapies. Ajuba is a member of the LIM domain family of proteins whose expression is positively associated with numerous cancers. Our data shows that Ajuba is highly expressed in human colon cancer tissue and cell lines. Publicly available data from The Cancer Genome Atlas shows a negative correlation between survival and Ajuba expression in patients with colon cancer. To investigate its function, we transduced SW480 human colon cancer cells, with lentiviral constructs to knockdown or overexpress Ajuba protein. The transcriptome of the modified cell lines was analyzed by RNA sequencing. Among the pathways enriched in the differentially expressed genes, were cell proliferation, migration and differentiation. We confirmed our sequencing data with biological assays; cells depleted of Ajuba were less proliferative, more sensitive to irradiation, migrated less and were less efficient in colony formation. In addition, loss of Ajuba expression decreased the tumor burden in a murine model of colorectal metastasis to t...Continue Reading

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Citations

Dec 30, 2020·Experimental Dermatology·Krista Schleicher, Daniel Schramek
Jun 27, 2021·Journal of Cellular and Molecular Medicine·Zhaoxia WuHaixia Peng

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Datasets Mentioned

BETA
GSE147111

Methods Mentioned

BETA
RNA-seq
PCR
Protein Assay
fluorescence microscopy
FACS

Software Mentioned

premessa
R package ggkm
R package DESeq2
gProfileR2
HISAT2
ggplot2
ImageJ
Cytobank
R
SAMtools

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