The linkage between oxygenation and subunit dissociation in human hemoglobin. Consequences for the analysis of oxygenation curves.

Biochemistry
G K AckersS Shapiro

Abstract

For human hemoglobin, a pronounced dependence of oxygenation curves upon protein concentration can be demonstrated experimentally in the range between 10(-4) and 2 X 10(-6) M heme. The effects of such protein concentration dependence upon analysis of saturation curves have been explored using a model-independent linkage analysis which incorporates the dissociation of tetramers to dimers. We have carried out stimulations of oxygenation curves representing a variety of energy distributions designed to cover a wide range of values which are relevant to known hemoglobin systems and experimental conditions. The resulting simulated oxygenation curves were analyzed by least-squares minimization procedures in terms of the tetramer binding isotherm to yield the four apparent Adair constants. These derived constants were compared with the originally assumed values used in the simulation in order to assess the extent to which their values may be altered by the presence of dimer. For each energy distribution the analysis has been carried out over a wide range of protein concentration. We have found that the presence of even small amounts of dimer that are necessarily present at the low protein concentrations commonly employed may have a de...Continue Reading

References

Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A P Minton, K Imai
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Citations

Jan 25, 1977·Biochimica Et Biophysica Acta·K Imai, H Yonetani
May 24, 1978·Biochimica Et Biophysica Acta·J V KilmartinJ M Baldwin
Jan 1, 1985·Comparative Biochemistry and Physiology. A, Comparative Physiology·P Vorger, D Matelin
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R ValdesG K Ackers
Dec 24, 2010·Molecular Biology of the Cell·Ivan NovotnýJan Malinsky
May 20, 1986·Journal of Molecular Biology·A CupaneL Cordone
Apr 1, 1977·Analytical Biochemistry·J D MahoneyS J Gill
Sep 15, 1988·European Journal of Biochemistry·H Schönert, B Stoll
Dec 1, 1978·Archives of Biochemistry and Biophysics·F Womack, S P Colowick
Apr 18, 1978·Biochemistry·M L Johnson, D A Yphantis
Jul 25, 1978·Journal of Molecular Biology·J V Kilmartin, N L Anderson
Mar 8, 1976·Biochemical and Biophysical Research Communications·G K AckersS H IP

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