The linker histone plays a dual role during gametogenesis in Saccharomyces cerevisiae.

Molecular and Cellular Biology
Jessica M BryantShelley L Berger

Abstract

The differentiation of gametes involves dramatic changes to chromatin, affecting transcription, meiosis, and cell morphology. Sporulation in Saccharomyces cerevisiae shares many chromatin features with spermatogenesis, including a 10-fold compaction of the nucleus. To identify new proteins involved in spore nuclear organization, we purified chromatin from mature spores and discovered a significant enrichment of the linker histone (Hho1). The function of Hho1 has proven to be elusive during vegetative growth, but here we demonstrate its requirement for efficient sporulation and full compaction of the spore genome. Hho1 chromatin immunoprecipitation followed by sequencing (ChIP-seq) revealed increased genome-wide binding in mature spores and provides novel in vivo evidence of the linker histone binding to nucleosomal linker DNA. We also link Hho1 function to the transcription factor Ume6, the master repressor of early meiotic genes. Hho1 and Ume6 are depleted during meiosis, and analysis of published ChIP-chip data obtained during vegetative growth reveals a high binding correlation of both proteins at promoters of early meiotic genes. Moreover, Ume6 promotes binding of Hho1 to meiotic gene promoters. Thus, Hho1 may play a dual r...Continue Reading

References

Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·C M Steber, R E Esposito
Feb 1, 1997·Yeast·S C UshinskyR K Storms
Oct 23, 1998·Science·S ChuI Herskowitz
Jun 18, 1999·Methods in Enzymology·P D Gregory, W Hörz
Dec 10, 1999·The Plant Cell·M Prymakowska-BosakA Jerzmanowski
Dec 2, 2000·Nature Genetics·M PrimigR E Esposito
Mar 30, 2001·The Journal of Biological Chemistry·K HellauerB Turcotte
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Roy M WilliamsRochelle E Esposito
May 2, 2003·International Review of Cytology·Yona KassirGalit Shenhar
Jun 25, 2003·Molecular Cell·Jessica A DownsStephen P Jackson
Aug 20, 2004·European Journal of Biochemistry·Jérôme GovinSaadi Khochbin
Jul 2, 2005·Biochimie·Akash GunjanAlain Verreault
Apr 4, 2006·Genetics·Marie VeronPierre-Antoine Defossez
Nov 15, 2006·Methods : a Companion to Methods in Enzymology·Kelby O KizerBrian D Strahl
Sep 25, 2007·Molecular Cell·Michael J MalloryRandy Strich
Oct 2, 2007·Genome Biology·Rod Balhorn
Jan 23, 2008·Biological Chemistry·Annalisa IzzoRobert Schneider
Aug 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Anat LevyDon Jay Katcoff
Sep 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Georgia SchäferHugh-G Patterton
Feb 28, 2009·The International Journal of Developmental Biology·James S Godde, Kiyoe Ura
May 5, 2009·The International Journal of Developmental Biology·Jerome Govin, Shelley L Berger
Jun 16, 2009·Nature·Saher Sue HammoudBradley R Cairns
Aug 27, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·José M Eirín-López, Juan Ausió
Oct 17, 2009·The EMBO Journal·Ulrich BraunschweigBas van Steensel
May 18, 2010·Nature Structural & Molecular Biology·Urszula BrykczynskaAntoine H F M Peters
Aug 18, 2010·Genes & Development·Jérôme GovinShelley L Berger
Nov 16, 2010·Developmental Cell·Satya K Kota, Robert Feil
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Aurélie LardenoisMichael Primig

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Citations

Sep 12, 2013·Oxidative Medicine and Cellular Longevity·Katya UzunovaGeorge Miloshev
Oct 17, 2013·PloS One·Hideaki TakataKazuhiro Maeshima
Dec 23, 2014·Chromosoma·Corinna LielegPhilipp Korber
Aug 12, 2014·Frontiers in Plant Science·Wenjing She, Célia Baroux
Dec 30, 2014·The International Journal of Biochemistry & Cell Biology·Milena GeorgievaGeorge Miloshev
Oct 21, 2015·Biochimica Et Biophysica Acta·Lluís Millán-AriñoAlbert Jordan
Jul 29, 2015·Molecular and Cellular Biology·Liang JinAaron M Neiman
Nov 21, 2019·Andrologia·Zhaohui ZhangXianjiang Kang
Jan 28, 2021·ELife·Nikhil R BhagwatNeil Hunter

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