The lipid alterations in the stratum corneum of dogs with atopic dermatitis are alleviated by topical application of a sphingolipid-containing emulsion

Clinical and Experimental Dermatology
Iuliana PopaJacques Portoukalian

Abstract

Atopic dermatitis (AD) results from an altered skin barrier associated with defects in the lipid composition of the skin. Dogs with AD present similar clinical symptoms to humans, and may be a useful model for investigations into AD. To analyse the changes occurring in the lipids of the stratum corneum (SC) of dogs with AE after 3 weeks of topical treatment with an emulsion containing ceramides, free fatty acids (FFAs) and cholesterol (skin lipid complex; SLC). Nonlesional SC was collected by tape stripping from control and treated areas. Free and protein-bound lipids were purified, and the various classes were isolated by column chromatography, analysed by thin-layer chromatography and assayed. Ceramides, FFA and cholesterol were all found to be lower in the skin of untreated dogs with AD than in normal dogs, and the topical treatment resulted in significantly increased values for ceramides. Conversely, only trace amounts of glucosylceramides were present in normal SC, but a high concentration (27 μg per mg protein) was detected in canine atopic SC, which disappeared after treatment with SLC. There was a heterogeneous distribution of all of the lipids in the different layers of canine atopic SC, which was more pronounced for p...Continue Reading

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Citations

Aug 16, 2015·BMC Veterinary Research·Thierry OlivryUNKNOWN International Committee on Allergic Diseases of Animals
Jan 22, 2013·Veterinary Dermatology·Rosanna Marsella
Sep 23, 2014·The British Journal of Dermatology·M Haftek
Nov 20, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Sumit PaliwalSamir Mitragotri
May 9, 2019·Journal of the American Veterinary Medical Association·Timothy J NuttallValerie A Fadok
Mar 22, 2020·BMC Veterinary Research·Rosanna MarsellaLluís Ferrer
Oct 24, 2017·Veterinary Sciences·Rosanna Marsella, Anna De Benedetto
Feb 26, 2013·The Veterinary Record·Tim NuttallRichard Halliwell
Nov 28, 2017·Biochimica Et Biophysica Acta. Biomembranes·Suttiwee ChermprapaiJoke A Bouwstra

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Atopic dermatitis is a chronic inflammatory genetically determined disease of the skin marked by increased ability to form reagin (IgE), with increased susceptibility to allergic rhinitis and asthma, and hereditary disposition to a lowered threshold for pruritus. Discover the latest research on atopic dermatitis here.