The lipopolysaccharide (LPS) of Photorhabdus luminescens TT01 can elicit dose- and time-dependent immune priming in Galleria mellonella larvae

Journal of Invertebrate Pathology
Gongqing WuLihong Qiu

Abstract

In this work, we primed Galleria mellonella larvae by haemocoel injection of lipopolysaccharide (LPS) extracted from Photorhabdus luminescens TT01 to determine whether bacterial LPS can induce enhanced immune protection (recently called immune priming). We also analyzed the relationship between changes in the levels of innate immune elements and the degree of enhanced immune protection in the larvae at designated time points after priming. The larvae that received experimental doses (20.0, 10.0 and 5.0μg per larva) of LPS demonstrated increased resistance against lethal challenge with P. luminescens TT01; the degree and period of protection correlated positively with the priming dose. These results indicated that the P. luminescens TT01 LPS could induce typical immune priming in G. mellonella. Moreover, the levels of innate immune parameters (i.e. haemocyte density, phagocytosis, haemocyte encapsulation ability, and antibacterial activity of cell-free haemolymph) and endogenous enzyme activities (i.e. acid phosphatase, ACP; alkaline phosphatase, AKP; superoxide dismutase, SOD and lysozyme, LSZ) were significantly increased following priming of the larvae with LPS, whereas the activities of peroxidase (POD) and catalase (CAT) we...Continue Reading

References

Jan 1, 1990·Free Radical Biology & Medicine·S Ahmad, R S Pardini
Jan 1, 1995·Archives of Insect Biochemistry and Physiology·S Ahmad
Dec 1, 1999·Developmental and Comparative Immunology·A E Halwani, G B Dunphy
Feb 13, 2001·Insect Biochemistry and Molecular Biology·C Lowenberger
Sep 13, 2002·Insect Biochemistry and Molecular Biology·X-Q YuM R Kanost
Apr 15, 2003·Nature Immunology·François LeulierBruno Lemaitre
Jun 14, 2003·Journal of Insect Physiology·S Asgari, O Schmidt
Oct 7, 2003·Nature Biotechnology·Eric DuchaudFrank Kunst
Oct 20, 2004·Journal of Invertebrate Pathology·P Korner, P Schmid-Hempel
Jun 20, 2006·Current Biology : CB·Ben M Sadd, Paul Schmid-Hempel
Dec 30, 2006·Peptides·Małgorzata CytryńskaTeresa Jakubowicz
Mar 14, 2007·PLoS Pathogens·Linh N PhamDavid S Schneider
Nov 6, 2007·Developmental and Comparative Immunology·Boran AltincicekAndreas Vilcinskas
Apr 11, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·I M DubovskiyV V Glupov
Sep 18, 2008·Proceedings. Biological Sciences·Olivia RothJoachim Kurtz
Apr 7, 2009·Microbes and Infection·Bernard CharrouxJulien Royet
Nov 26, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mayur K KajlaSusan M Paskewitz
Dec 19, 2009·The Journal of Biological Chemistry·Jianmin MengDouglas T Golenbock
Jun 19, 2010·Developmental and Comparative Immunology·Pawel MakMałgorzata Cytryńska
Jun 19, 2012·Biochimica Et Biophysica Acta·Agnieszka Zdybicka-BarabasMałgorzata Cytryńska
Dec 25, 2012·Biochemical and Biophysical Research Communications·Bryce NelsonHirotaka Kanuka
Feb 20, 2013·Biochimica Et Biophysica Acta·Agnieszka Zdybicka-BarabasMałgorzata Cytryńska
Dec 3, 2013·Microbes and Infection·J Contreras-GarduñoH Lanz-Mendoza
Apr 8, 2014·Cellular Signalling·Valluri V SatyavathiJavaregowda Nagaraju

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Citations

Jul 13, 2016·Seminars in Immunology·Barbara Milutinović, Joachim Kurtz
Jun 29, 2016·Zoology : Analysis of Complex Systems, ZACS·Barbara MilutinovićJoachim Kurtz
Mar 12, 2019·Future Microbiology·José A Martínez-ÁlvarezHéctor M Mora-Montes
Sep 7, 2018·Frontiers in Immunology·Daniela MelilloDiana Boraschi
Feb 6, 2016·Insect Science·Iwona Wojda
Sep 2, 2020·Journal of Fungi·Laura C García-CarneroHéctor M Mora-Montes
Mar 5, 2021·Journal of Insect Physiology·Gerard SheehanKevin Kavanagh
Mar 11, 2021·Microbial Pathogenesis·Méndez-López Texca TatevariRamirez-Romero Ricardo
Sep 1, 2021·Journal of Invertebrate Pathology·Michał SułekIwona Wojda
Aug 29, 2021·Developmental and Comparative Immunology·Cybèle Prigot-MauriceChristine Braquart-Varnier

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