PMID: 16505592Mar 1, 2006Paper

The lipoprotein fraction between VLDL and LDL detected by biphasic agarose gel electrophoresis reflects serum remnant lipoprotein and Lp(a) concentrations

Journal of Atherosclerosis and Thrombosis
Itsuko SatoShunichi Kumagai

Abstract

We analyzed lipoprotein profiles in 616 Japanese by biphasic agarose gel electrophoresis using Chol/Trig Combo(TM) to yield HDL, VLDL, LDL and CM fractions which were stained with cholesterol and triglyceride reagents, respectively. To further evalute the pattern of electrophoresis, we analyzed the fraction between VLDL and LDL to confirm the possibility of a MidBand by using an automatic-five-fraction function. The cholesterol concentrations in MidBand (MidBand-C) showed a good correlation to remnant-like particle-cholesterol (RLP-C) (r = 0.95) in 23 consecutive samples (TC < 220 mg/dl, Lp(a) < 30 mg/dl). However, MidBand-C concentrations of subjects with high Lp(a) levels (Lp(a) > 30 mg/dl) were also high compared to RLP-C concentrations. The average MidBand-C levels in elderly normolipidemic control subjects (TC < 220, TG < 150) were 5.2 +/- 2.4 mg/dl in 30 males (mean age, 70 +/- 10 years) and 5.4 +/- 2.0 mg/dl in 40 females (64 +/- 11 years). The average MidBand-C levels of normolipidemic patients with coronary artery diseases (CAD; TC < 220, TG < 150) were 9.4 +/- 4.1 mg/dl in 126 males (mean age, 66 +/- 10 years) and 9.1 +/- 4.0 mg/dl in 44 females (67 +/- 10 years). These levels were significantly higher than control va...Continue Reading

References

Apr 1, 1994·Current Opinion in Lipidology·R J Havel
Jun 24, 2000·Annals of the New York Academy of Sciences·S KawasakiM Yokoyama

❮ Previous
Next ❯

Citations

Dec 8, 2009·Journal of Lipid Research·Yuji HirowatariNorio Tada
Mar 6, 2009·Journal of Atherosclerosis and Thrombosis·Itsuko SatoShunichi Kumagai
Jul 28, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shigetoshi HosakaKazutoshi Maruyama
Nov 18, 2006·Clinics in Laboratory Medicine·G Russell WarnickCleland C Landolt
Dec 26, 2006·Circulation Journal : Official Journal of the Japanese Circulation Society·Tomohiko YasunagaShigeru Kohno
May 22, 2021·Frontiers in Oncology·Takatoshi AnnoHideaki Kaneto

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.