The Listeria monocytogenes Bile Stimulon under Acidic Conditions Is Characterized by Strain-Specific Patterns and the Upregulation of Motility, Cell Wall Modification Functions, and the PrfA Regulon

Frontiers in Microbiology
Veronica Guariglia-OropezaKathryn J Boor

Abstract

Listeria monocytogenes uses a variety of transcriptional regulation strategies to adapt to the extra-host environment, the gastrointestinal tract, and the intracellular host environment. While the alternative sigma factor SigB has been proposed to be a key transcriptional regulator that facilitates L. monocytogenes adaptation to the gastrointestinal environment, the L. monocytogenes' transcriptional response to bile exposure is not well-understood. RNA-seq characterization of the bile stimulon was performed in two L. monocytogenes strains representing lineages I and II. Exposure to bile at pH 5.5 elicited a large transcriptomic response with ~16 and 23% of genes showing differential transcription in 10403S and H7858, respectively. The bile stimulon includes genes involved in motility and cell wall modification mechanisms, as well as genes in the PrfA regulon, which likely facilitate survival during the gastrointestinal stages of infection that follow bile exposure. The fact that bile exposure induced the PrfA regulon, but did not induce further upregulation of the SigB regulon (beyond that expected by exposure to pH 5.5), suggests a model where at the earlier stages of gastrointestinal infection (e.g., acid exposure in the stom...Continue Reading

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Citations

Sep 6, 2019·Microbiology and Molecular Biology Reviews : MMBR·Ahmed GaballaKathryn J Boor
Sep 26, 2019·Frontiers in Microbiology·Atsadang BoonmeeSoraya Chaturongakul
Mar 31, 2021·Critical Reviews in Food Science and Nutrition·Karolinny Cristiny de Oliveira VieiraLizziane Kretli Winkelstroter Eller
Dec 7, 2021·Frontiers in Microbiology·Damayanti ChakravartyJanet R Donaldson

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Datasets Mentioned

BETA
GSE103443

Methods Mentioned

BETA
chip
RNA-seq
phosphotransferase

Software Mentioned

BlastN
GOseq
samtools
R
BLAST
Bayseq
BWA
Bayseq package R

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