The lncRNA MALAT1/miR-30/Spastin Axis Regulates Hippocampal Neurite Outgrowth

Frontiers in Cellular Neuroscience
Tao JiangMinghui Tan

Abstract

Spastin, a microtubule-severing enzyme, is important for neurite outgrowth. However, the mechanisms underlying the post-transcriptional regulation of spastin during microtubule-related processes are largely unknown. We demonstrated that the spastin expression level is controlled by a long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1)/microRNA-30 (miR-30) axis during neurite outgrowth. The miR-30 expression level decreased in hippocampal neurons with increasing days in culture, and miR-30 overexpression suppressed while miR-30 inhibition promoted neurite outgrowth in hippocampal neurons. Spastin was validated as a target gene of miR-30 using the luciferase reporter assay. The protein expression, microtubule severing activity, and neurite promoting effect of spastin were suppressed by the overexpression of miR-30 mimics and increased by miR-30 inhibitors. MALAT1 expression increased during neurite outgrowth and MALAT1 silencing impaired neurite outgrowth. miR-30 was a sponge target of MALAT1 and MALAT1/miR-30 altered neurite outgrowth in hippocampal neurons. MALAT1 overexpression reversed the inhibitory effect of miR-30 on the activity of a luciferase reporter construct containing spastin...Continue Reading

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Citations

Apr 4, 2021·International Journal of Molecular Sciences·Hanna Sas-Nowosielska, Adriana Magalska
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sarva KeihaniEugenio F Fornasiero

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Methods Mentioned

BETA
Assay
transfection
transfect
PCR
protein assay

Software Mentioned

Pro Plus
Image
picTar
miRDB
TargetScan
GraphPad Prism

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