The lncRNA TUG1/miR-145-5p/FGF10 regulates proliferation and migration in VSMCs of hypertension

Biochemical and Biophysical Research Communications
Lin ShiZhaoyang Meng

Abstract

Vascular remodeling is a characteristic pathological feature of hypertension, it can cause of increasing vascular resistance and decrease of compliance. Vascular smooth muscle cell (VSMCs) dysfunction is the important foundation of vascular remodeling. Increasing evidences have revealed that lncRNA is an important regulatory factor of VSMC function. In this paper, we explored the function of lncRNA TUG1 in vascular remodeling of hypertension. Here, we found that lncRNA TUG1 was highly expressed in aorta of spontaneously hypertensive rats (SHR) rats and promoted the proliferation and migration of VSMCs (SHR-VSMCs). Bioinformatics analyze showed that lncRNA TUG1 sequence had miR-145-5p binding sites. Luciferase reporter test, RNA pulldown and qRT-PCR showed that lncRNA TUG1 could bind miR-145-5p. Similarly, bioinformatics analyze found that FGF10 3 'UTR contained miR-145-5p binding sites. Luciferase reporter test, qRT-PCR and Western blot were shown that miR-145-5p inhibited FGF10 expression by binding to its 3 'UTR. MTT showed that miR-145-5p inhibited and FGF10 promoted SHR-VMSCs proliferation and migration. Overexpression of miR-145-5p or knocking down of FGF10 after overexpresion of lncRNA TUG1 could rescue the proliferation ...Continue Reading

Citations

May 21, 2019·Technology in Cancer Research & Treatment·Baoying WangXiaojie Li
Aug 12, 2020·Journal of Cell Communication and Signaling·Jingbo ChenChaohong Li
Sep 29, 2020·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Ji-Ru Zhang, Hai-Jian Sun
Nov 9, 2018·Frontiers in Genetics·Joanne Watson, Chiara Francavilla
Jul 30, 2019·BioMed Research International·Huan ZhouJun-Ping Zhang
Jan 25, 2020·Journal of Bone and Mineral Metabolism·Xue ZhangQiu-Hua Liang
Feb 6, 2020·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Xiaoying Jiang, Qilan Ning
Nov 21, 2020·Journal of Cellular and Molecular Medicine·Chaowei HuYongxiang Wei
Jan 15, 2021·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Federica ReyStephana Carelli
Feb 6, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shun ZhangWujun Chen
Jan 30, 2021·Frontiers in Cell and Developmental Biology·Dongdong ZhengKexiang Liu
Feb 23, 2021·Biochemical Society Transactions·Kirsten Riches-Suman
Feb 24, 2020·International Immunopharmacology·Palani DineshMahaboobkhan Rasool
Jul 10, 2020·The Journal of Nutritional Biochemistry·Haixia LinKelly E Mercer
Apr 24, 2021·Frontiers in Cardiovascular Medicine·Yaoyao GuoGang Yuan
May 28, 2021·Frontiers in Cardiovascular Medicine·Jiawen LiYifei Li
Jun 29, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Lee OhayonPartha Dutta
Sep 22, 2021·Animal Science Journal = Nihon Chikusan Gakkaihō·Xiaoli XuJiaxue Cao
Oct 30, 2021·Molecular Medicine Reports·Yunyu LiangPingchang Xie

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.