PMID: 2125076Sep 1, 1990Paper

The local anaesthetic tetracaine as a quencher of perylene fluorescence in micelles

Journal of Photochemistry and Photobiology. B, Biology
M S Fernández, E Calderón

Abstract

At neutral pH the local anaesthetic tetracaine hydrochloride quenches the fluorescence of the lipophilic dye perylene incorporated into non-ionic micelles. The process follows the Stern-Volmer equation, suggesting that quenching occurs through encounter of fluorophore and quencher. As the pH is lowered from 5 to 1, the apparent quenching constant decreases sigmoidally, the midpoint of the curve being at pH 2.3, close to the pK value characterizing the ionization of the anaesthetic aromatic butylamino group. Quenching is completely reversed below pH 1. These results show that the ability of tetracaine to quench the fluorescence of perylene incorporated into micelles depends on the absence of charge on its aromatic amine. Quenching was also studied in homogeneous dioxane-water solution. In this system the quenching constant also decreases sigmoidally as the pH is lowered. The infection point of the curve is nearly coincident with the pK of tetracaine butylamino group in the same partially non-aqueous medium. Protonation of this group induces 60% reversal of the quenching, suggesting that the main mechanism of fluorescence extinction could be the electron transfer from unprotonated tetracaine aromatic amine to perylene in the exci...Continue Reading

References

Nov 1, 1975·Archives of Biochemistry and Biophysics·D D KoblinH H Wang
Feb 1, 1976·Archives of Biochemistry and Biophysics·M S Fernández, J Cerbón
May 1, 1973·Archives of Biochemistry and Biophysics·G Papageorgiou, C Argoudelis
Apr 1, 1984·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·E C Kelusky, I C Smith
Jun 10, 1983·Biochimica Et Biophysica Acta·J García-Soto, M S Fernández
Mar 8, 1982·Biochimica Et Biophysica Acta·J WestmanI C Smith
Aug 6, 1981·Biochimica Et Biophysica Acta·M S Fernández
Nov 1, 1964·The Journal of General Physiology·M B FEINSTEIN

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Citations

Jul 30, 2005·Cell Death and Differentiation·S B LeS H Kaufmann
Oct 22, 2013·International Journal of Nanomedicine·Aurora MocanuMaria Tomoaia-Cotisel

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