The localization of mcalpain in myelin: immunocytochemical evidence in different areas of rat brain and nerves

Brain Research
Z Li, N L Banik

Abstract

A major part of brain mcalpain activity has been found associated with myelin, but its presence in the myelin sheath has not been clearly demonstrated by microscopic (morphological) means. Using myelin mcalpain antisera the localization of mcalpain has been investigated in tissue of rat CNS and PNS by immunohistochemical methods. These experiments also have been carried out by double labeling studies using antibodies to myelin basic protein (MBP) and neurofilament protein (NFP). Our results indicate calpain/MBP immunoreactivity in the myelin sheath surrounding the axon while NFP antibody stained inside the axon in spinal cord; pons, cerebellum, trigeminal nerve, and sciatic nerve. Patches of light immunoreactivity of calpain were also seen in the axonal cytoplasm. The calpain immunostaining of myelin was similar to that of MBP staining indicating the presence of calpain in myelin. This finding supports the view that calpain is a constituent of myelin, may be involved in the normal turnover of myelin proteins. In pathological situations such as in demyelinating and other brain degenerative diseases, myelin may be autodigestive.

References

Jan 1, 1979·Journal of Neurochemistry·W W Schlaepfer, S Micko
Jun 1, 1992·Molecular and Chemical Neuropathology·K Domańska-JanikT Zalewska
Nov 15, 1991·Brain Research·M MataD J Fink
Sep 1, 1988·Journal of Neurochemistry·K YanagisawaT Miyatake
Nov 1, 1989·Neurochemical Research·K Blomgren, J O Karlsson
May 13, 1988·Science·J E Schollmeyer
Jul 1, 1988·Journal of Neuroscience Research·A K ChakrabartiN L Banik
May 14, 1986·Biochemical and Biophysical Research Communications·Y AdachiM Hatanaka
Jan 1, 1986·Journal of Neurobiology·M BaudryG Lynch
Apr 14, 1970·Brain Research·P J RiekkinenA U Arstila
Nov 1, 1968·Journal of Neurochemistry·C S BeckM E Smith
Jun 8, 1984·Science·G Lynch, M Baudry
Aug 19, 1983·Neuroscience Letters·I Singh, A K Singh
May 1, 1994·Neurochemical Research·M Banay-SchwartzA Lajtha
Jan 15, 1993·Brain Research. Developmental Brain Research·A K ChakrabartiE L Hogan
Jun 1, 1963·Journal of Neurochemistry·C W ADAMSN A GREGSON

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Citations

Dec 13, 2018·Neural Regeneration Research·Jamileh DibasAdnan Dibas
Sep 25, 2002·Physiological Reviews·Linda R Watkins, Steven F Maier
Oct 7, 2010·Molecular Neurobiology·Sohila ZadranMichel Baudry
Oct 31, 2012·Journal of Neurochemistry·Arabinda DasNaren L Banik
Oct 19, 2006·Journal of Neuro-oncology·Hyang-Hwa RyuSam-Suk Kang
Nov 23, 2000·Journal of Neuropathology and Experimental Neurology·M R Del Bigio
Sep 25, 1997·Journal of Neuroscience Research·M C SubangP M Richardson
Dec 28, 1999·Brain Research. Brain Research Reviews·M A Petty, J G Wettstein
Aug 23, 2003·Brain Research·Masayuki YokotaTakashi Watanabe

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