The localization of plasminogen activator inhibitor-1 in glomerular subepithelial deposits in membranous nephropathy.

Journal of the American Society of Nephrology : JASN
T NakamuraS Yokota

Abstract

In this study, the localization of plasminogen activator inhibitor-1 (PAI-1) and its association with glomerular subepithelial deposits of vitronectin (VN) in membranous nephropathy (MN) was evaluated. Renal biopsy tissue from 30 patients with MN along with specimens of normal kidney removed from six patients with renal tumors were examined for glomerular deposits of urokinase-type plasminogen activator, tissue-type plasminogen activator (t-PA), PAI-1, VN, and fibrinogen by using immunofluorescence and immunoelectron microscopic techniques. Deposits were characterized by pretreating the frozen sections with arginine, glycine, and t-PA to dissociate the VN-PAI-1 complexes, thus providing evidence of PAI-1 binding to VN. Glomeruli isolated from normal frozen kidney and from frozen kidney tissue with MN were subjected to immunoblot analysis. VN was found in the mesangial area and t-PA was observed along the capillary wall of normal glomeruli. Coarse granular deposits of VN, PAI-1, and t-PA were observed along the glomerular basement membrane in MN. Immunoelectron microscopy showed the presence of VN, PAI-1, and t-PA in the subepithelial immune deposits. Pretreating the samples with arginine decreased or abolished immunofluorescenc...Continue Reading

Citations

May 16, 2000·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·L TarnowH H Parving
Feb 22, 2003·Journal of the American Society of Nephrology : JASN·Hitomi KawanoHiroshi Iwao
Oct 13, 2006·Journal of the American Society of Nephrology : JASN·Allison A Eddy, Agnes B Fogo
Dec 1, 2001·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·P HovindH H Parving
Mar 29, 2002·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Kazumasa HamanoKazuhiro Dohi
Mar 27, 2009·Nature Reviews. Nephrology·Hunjoo HaHi B Lee
Feb 1, 2011·Protein Science : a Publication of the Protein Society·Lawrence C ThompsonCynthia B Peterson
Jul 6, 2011·Nature Reviews. Nephrology·Toshio MiyataCharles van Ypersele de Strihou

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