The location of acidic fibroblast growth factor in the breast is dependent on the activity of proteases present in breast cancer tissue

British Journal of Cancer
R CoopeJ J Gomm

Abstract

Acidic fibroblast growth factor (FGF1) and two of its receptors, FGFR1 and FGFR4, were localized in cryostat sections of normal, benign and malignant human breast tissue by immunohistochemistry. Without pretreatment, FGF1 staining was mainly seen in normal epithelial cells. However, polymerase chain reaction (PCR) analysis and immunoblotting of isolated normal epithelial and myoepithelial cells showed FGF1 mRNA and protein to be present in both cell types. Following incubation of frozen sections at 37 degrees C in phosphate-buffered saline, FGF1 staining was also revealed in myoepithelial cells and basement membrane adjacent to carcinoma cells. Treatment with protease inhibitors demonstrated that this effect was due to the activity of an endogenous protease. In contrast, FGF1 staining was found to be associated with the stroma adjacent to malignant cells only in the presence of protease inhibitors. FGFR1 and FGFR4 immunostaining was localized to both normal and malignant epithelial cells and to a lesser extent to myoepithelial cells. There was no difference in the staining intensity for the FGF receptors between normal and cancer samples. The change in location of FGF1 between normal and malignant tissues and the sensitivity of...Continue Reading

Citations

Mar 16, 2001·Breast Cancer Research : BCR·C DicksonV Fantl
Oct 25, 2002·American Journal of Respiratory Cell and Molecular Biology·Andor R KranenburgHari S Sharma
Mar 5, 1999·Breast Cancer Research and Treatment·N LocopoG Gasparini
Jan 8, 2004·British Journal of Cancer·P JézéquelG Ricolleau
Sep 3, 1999·Breast Cancer Research and Treatment·A M SieuwertsJ A Foekens
Feb 13, 2001·Critical Reviews in Oncology/hematology·G Gasparini

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