The Long Non-coding RNA Cyrano Is Dispensable for Pluripotency of Murine and Human Pluripotent Stem Cells.

Stem Cell Reports
Hannah J HunklerChristian Bär

Abstract

Pluripotency is tightly regulated and is crucial for stem cells and their implementation for regenerative medicine. Non-coding RNAs, especially long non-coding RNAs (lncRNAs) emerged as orchestrators of versatile (patho)-physiological processes on the transcriptional and post-transcriptional level. Cyrano, a well-conserved lncRNA, is highly expressed in stem cells suggesting an important role in pluripotency, which we aimed to investigate in loss-off-function (LOF) experiments. Cyrano was described previously to be essential for the maintenance of mouse embryonic stem cell (ESC) pluripotency. In contrast, using different genetic models, we here found Cyrano to be dispensable in murine and human iPSCs and in human ESCs. RNA sequencing revealed only a moderate influence of Cyrano on the global transcriptome. In line, Cyrano-depleted iPSCs retained the potential to differentiate into the three germ layers. In conclusion, different methods were applied for LOF studies to rule out potential off-target effects. These approaches revealed that Cyrano does not impact pluripotency.

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Citations

Mar 23, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pei LuWei Jiang
Mar 24, 2021·Wiley Interdisciplinary Reviews. RNA·Elena Rivas
May 26, 2021·PLoS Genetics·Kazutoshi TakahashiShinya Yamanaka
Jan 19, 2022·Clinical and Translational Medicine·Serena Wooten, Keriayn N Smith

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Datasets Mentioned

BETA
GSE150421

Methods Mentioned

BETA
confocal microscopy
FACS
PCR
Flow cytometry
Fluorescence
RNA-seq
Assay
electrophoresis

Software Mentioned

Cyrano
FlowJo
LAS X
Leica
DEseq2

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