Abstract
Pluripotency is tightly regulated and is crucial for stem cells and their implementation for regenerative medicine. Non-coding RNAs, especially long non-coding RNAs (lncRNAs) emerged as orchestrators of versatile (patho)-physiological processes on the transcriptional and post-transcriptional level. Cyrano, a well-conserved lncRNA, is highly expressed in stem cells suggesting an important role in pluripotency, which we aimed to investigate in loss-off-function (LOF) experiments. Cyrano was described previously to be essential for the maintenance of mouse embryonic stem cell (ESC) pluripotency. In contrast, using different genetic models, we here found Cyrano to be dispensable in murine and human iPSCs and in human ESCs. RNA sequencing revealed only a moderate influence of Cyrano on the global transcriptome. In line, Cyrano-depleted iPSCs retained the potential to differentiate into the three germ layers. In conclusion, different methods were applied for LOF studies to rule out potential off-target effects. These approaches revealed that Cyrano does not impact pluripotency.
References
Aug 15, 2006·Cell·Kazutoshi Takahashi, Shinya Yamanaka
Nov 23, 2007·Blood·Richard P DavisEdouard G Stanley
Dec 27, 2011·Cell·Igor UlitskyDavid P Bartel
Jan 4, 2012·Stem Cells·Josipa Bilic, Juan Carlos Izpisua Belmonte
Oct 30, 2012·European Heart Journal·George KensahUlrich Martin
Mar 26, 2013·Experimental Eye Research·Francis Beby, Thomas Lamonerie
Jul 16, 2013·Combinatorial Chemistry & High Throughput Screening·Bhavneet Bhinder, Hakim Djaballah
Feb 18, 2014·Molecular Cell·Nianwei LinTariq M Rana
Jun 24, 2014·Nature Biotechnology·Dirk HecklBenjamin L Ebert
Aug 13, 2015·ELife·Vikram AgarwalDavid P Bartel
Jan 28, 2016·Science·Benjamin R NelsonEric N Olson
Jan 29, 2016·Nucleic Acids Research·Jiyoung KimMyriam Gorospe
Mar 15, 2016·Cell Stem Cell·Mohammad A MandegarBruce R Conklin
Apr 26, 2016·Nature Biotechnology·Bastiaan EversRene Bernards
May 10, 2016·Nature Biotechnology·David W MorgensMichael C Bassik
Aug 19, 2016·Physiological Reviews·Julia BeermannThomas Thum
Nov 9, 2016·Circulation·Christian BärThomas Thum
Jan 4, 2017·Nature Reviews. Genetics·Mo Li, Juan Carlos Izpisua Belmonte
Jun 6, 2017·Stem Cell Reports·Keriayn N SmithTerry Magnuson
Jun 12, 2018·Cell·Benjamin KleavelandDavid P Bartel
Jan 9, 2019·ELife·Mehdi GoudarziAlexander F Schier
Jul 28, 2019·RNA·Marianna FeretzakiJoachim Lingner
Citations
Mar 23, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pei LuWei Jiang
Mar 24, 2021·Wiley Interdisciplinary Reviews. RNA·Elena Rivas
May 26, 2021·PLoS Genetics·Kazutoshi TakahashiShinya Yamanaka
Nov 1, 2021·Cardiovascular Research·Hannah J HunklerChristian Bär
Jan 19, 2022·Clinical and Translational Medicine·Serena Wooten, Keriayn N Smith