The long non-coding RNA landscape of periodontal ligament stem cells subjected to compressive force

European Journal of Orthodontics
Yiping HuangWeiran Li

Abstract

The role of long non-coding ribonucleic acids (lncRNAs) during orthodontic tooth movement remains unclear. We explored the lncRNA landscape of periodontal ligament stem cells (PDLSCs) subjected to compressive force. PDLSCs were subjected to static compressive stress (2 g/cm2) for 12 hours. Total RNA was then extracted and sequenced to measure changes in lncRNA and messenger RNA (mRNA) expression levels. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to validate the expression levels of certain lncRNAs. Differential expression analysis as well as Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were also performed. In total, 90 lncRNAs and 519 mRNAs were differentially expressed in PDLSCs under compressive stress. Of the lncRNAs, 72 were upregulated and 18 downregulated. The levels of eight lncRNAs of interest (FER1L4, HIF1A-AS2, MIAT, NEAT1, ADAMTS9-AS2, LUCAT1, MIR31HG, and DHFRP1) were measured via qRT-PCR, and the results were found to be consistent with those of RNA sequencing. GO and KEGG pathway analyses showed that a wide range of biological functions were expressed during compressive loading; most differentially expressed genes were involved in extracellular mat...Continue Reading

References

May 8, 2004·Nature Reviews. Genetics·John S Mattick
Apr 22, 2006·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Vinod Krishnan, Ze'ev Davidovitch
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Feb 23, 2013·Bioinformatics·Ning LengChristina Kendziorski
Mar 19, 2013·Cell·Pedro J Batista, Howard Y Chang
Jul 6, 2013·Cell·Igor Ulitsky, David P Bartel
Dec 29, 2013·Archives of Oral Biology·Rui Mauricio Santos de AraujoKeiji Moriyama
May 17, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jie YaoWeijie Zhu
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg
Jun 21, 2015·In Vitro Cellular & Developmental Biology. Animal·Maolin ChangGuangli Han
Jul 17, 2016·Journal of Dental Research·N ChenF Jin
Apr 19, 2017·The Angle Orthodontist·Ying-Ying ZhangYan Liu
May 19, 2017·Journal of Dental Research·M TakewakiH Kurihara
Jun 10, 2017·Journal of Periodontology·Yunfei ZhengWeiran Li
Sep 28, 2017·International Journal of Molecular Medicine·Dongru ChenJianqing Deng
Jan 23, 2018·Journal of Molecular Histology·Baiyu SunXin Xu

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.