The long non-coding RNA PCAL7 promotes prostate cancer by strengthening androgen receptor signaling.

Journal of Clinical Laboratory Analysis
Zhihui LiXing Ai

Abstract

The prostate cancer (PCa) has been a global problem to men health. Notably, the androgen receptor (AR) is essential for both normal development of prostate and prostate cancer progression. The RNA sequencing was used to identify the novel long non-coding RNA (lncRNA) termed PCAL7. The RT-qPCR was performed to quantify PCAL7 expression. Migration and proliferation assays were used to examine the function of PCAL7. Fluorescence in situ hybridization (FISH) was used to determine subcellular localization. By RNA sequencing, the differentially expressed lncRNAs were identified (top 10 upregulated lncRNAs: PCAL7, AC083843.1, CTC-338M12.3, RP11-443B7.1, RP11-1008C21.2, RN7SL329P, RP4-773N10.4, RP11-264B17.2, KB-1507C5.2, and RP11-20B24.6; top 10 downregulated lncRNAs: RP11-77H9.2, RAB11FIP1P1, AP001625.6, CTA-217C2.1, RP11-603J24.7, RP11-315I20.1, AC092839.1, RP4-758J18.10, RP11-259O2.3, and HMGN2P17). PCAL7 was the lncRNA with the highest fold upregulation and significantly correlated with AR signaling during prostate cancer progression. The AR-regulated PCAL7 was abundantly overexpressed in prostate cancer tissues and AR-dependent cell lines. PCAL7 knockdown inhibited cell migration and proliferation. Consistently, the migration and...Continue Reading

References

Mar 22, 2003·Current Opinion in Cell Biology·John J TysonBela Novak
Nov 3, 2004·Nature Reviews. Genetics·Hiroaki Kitano
Sep 17, 2010·Genes & Development·Michael M Shen, Cory Abate-Shen
Jul 5, 2011·Molecular Cell·Svetlana LebedevaNikolaus Rajewsky
Mar 28, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mark N SteinRobert S Dipaola
Aug 17, 2012·The New England Journal of Medicine·Howard I ScherUNKNOWN AFFIRM Investigators
Nov 22, 2014·Nature Communications·Dimple ChakravartyMark A Rubin
Mar 1, 2017·Nature Biotechnology·Stanley T CrookeXue-Hai Liang
Mar 2, 2017·The Journal of Clinical Investigation·Brian D AdamsFrank J Slack
Aug 18, 2018·Molecular Cancer Research : MCR·Shreyas LingadahalliEdwin Cheung
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 14, 2020·Neoplasia : an International Journal for Oncology Research·Steven KregelArul M Chinnaiyan

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Citations

Oct 17, 2021·Journal of Clinical Laboratory Analysis·Rubing LiFeng Wang

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Methods Mentioned

BETA
immunoprecipitation
antisense oligonucleotides
ChIP
RIP
xenografts
xenograft

Software Mentioned

SPSS

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