The long noncoding RNA GAS5 negatively regulates the adipogenic differentiation of MSCs by modulating the miR-18a/CTGF axis as a ceRNA

Cell Death & Disease
Ming LiHuiyong Shen

Abstract

Mesenchymal stem cells (MSCs) are important pluripotent stem cells and a major source of adipocytes in the body. However, the mechanism of adipogenic differentiation has not yet been completely elucidated. In this study, the long noncoding RNA GAS5 was found to be negatively correlated with MSC adipogenic differentiation. GAS5 overexpression negatively regulated adipocyte formation, whereas GAS5 knockdown had the opposite effect. Further mechanistic analyses using luciferase reporter assays revealed that GAS5 regulates the adipogenic differentiation of MSCs by acting as competing endogenous RNA (ceRNA) to sponge miR-18a, which promotes adipogenic differentiation. Mutation of the binding sites for GAS5 in miR-18a abolished the effect of the interaction. The miR-18a mimic and inhibitor reversed the negative regulatory effect of GAS5 on MSCs adipogenic differentiation. In addition, GAS5 inhibited miR-18a, which downregulates connective tissue growth factor (CTGF) expression, to negatively regulate the adipogenic differentiation of MSCs. Taken together, the results show that GAS5 serves as a sponge for miR-18a, inhibiting its capability to suppress CTGF protein translation and ultimately decreasing the adipogenic differentiation of...Continue Reading

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Citations

May 2, 2020·Stem Cells Translational Medicine·Yuanwei ChenLei Liu
Jul 2, 2019·International Journal of Molecular Medicine·Yingzheng WengLijiang Tang
Dec 26, 2018·Journal of Cellular Physiology·Xiaowen ChiWei Zhang
Oct 27, 2018·Journal of Cellular Physiology·Huiling LiangYuanbo Cui
Jan 8, 2019·BMC Medical Genetics·Michele SalemiAldo E Calogero
Apr 24, 2019·Diabetes·Lei Sun, Jiandie D Lin
Apr 6, 2019·World Journal of Stem Cells·Zhong-Yu XieHui-Yong Shen
Dec 11, 2020·International Journal of Molecular Sciences·Binglin YueHong Chen
May 1, 2021·International Journal of Molecular Sciences·Cinzia AuriliaMaria Luisa Brandi
Nov 10, 2021·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jiao LiXijie Yu

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Methods Mentioned

BETA
flow cytometry
transfection
electrophoresis
Assay
RIP
PCR
pull-down
immunoprecipitation

Software Mentioned

SPSS

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