The long repeat region is dispensable for fowl adenovirus replication in vitro

Virology
D Ojkic, Eva Nagy

Abstract

Two regions containing tandemly repeated sequences are present in the fowl adenovirus 9 (FAdV-9) genome. The longer repeat region (TR-2) is composed of 13 contiguous 135-bp-long direct repeats, the function of which is unknown. An infectious FAdV-9 genomic clone, constructed by homologous recombination in Escherichia coli, was used for engineering of recombinant viruses. The enhanced green fluorescence protein (EGFP) coding sequence was cloned in both rightward and leftward orientations so as to replace TR-2. Replication-competent recombinant FAdVs were recovered, demonstrating that TR-2 was dispensable for FAdV-9 propagation in vitro. The expression of EGFP in infected cells was demonstrated by fluorescence microscopy, immunoprecipitation, and RT-PCR.

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Citations

Jun 7, 2013·Clinical and Vaccine Immunology : CVI·Li DengEva Nagy
Feb 7, 2014·Human Gene Therapy·Estrella Lopez-GordoRamon Alemany
Jun 23, 2006·Virus Genes·Juan Carlos CorredorE Nagy
Jan 22, 2008·Virus Genes·Juan Carlos CorredorEva Nagy
Aug 5, 2015·Journal of Virological Methods·Yanlong PeiÉva Nagy
May 16, 2009·Journal of Virological Methods·Nadya RomanovaEva Nagy
Jul 21, 2015·Virology·Robert A KozakÉva Nagy
Nov 28, 2015·Avian Pathology : Journal of the W.V.P.A·Pedro F Vera-HernándezAngel E Absalón
Nov 22, 2005·Vaccine·Dinesh S Bangari, Suresh K Mittal
Nov 21, 2002·Virology·Davor OjkicEva Nagy
Oct 27, 2016·Canadian Journal of Microbiology·Patrick D SlaineÉva Nagy
Sep 18, 2009·The Journal of General Virology·Juan Carlos Corredor, Eva Nagy
Sep 25, 2019·Virus Genes·Yahiro MukaiMasayuki Horie

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