The long terminal repeats of human immunodeficiency virus type-1 and human T-cell leukemia virus type-I are activated by 12-O-tetradecanoylphorbol-13-acetate through different pathways

Virology
N Mor-VakninM Aboud

Abstract

The LTRs of HIV-1 and HTLV-I have been shown by several laboratories to be activated by 12-O-tetradecanoylphorbol-13-acetate (TPA). This agent is a potent activator of protein kinase C (PKC). However, long exposure to TPA downregulates PKC in many cell types. We demonstrated that TPA treatment of Jurkat cells for more than 24 hr resulted in a sever depletion of this enzyme. Therefore, to explore the role of PKC in the effect of TPA on these LTRs, we transfected Jurkat cells with HIV-1 LTR-CAT or HTLV-I LTR-CAT construct after 72 hr of TPA pretreatment. While this TPA pretreatment considerably reduced the HIV-1 LTR basal expression, it strongly stimulated the expression of HTLV-I LTR. Furthermore, when TPA was added after transfection, a strong stimulation of HIV-1 LTR was observed, which could be abrogated by PKC inhibitors like H7 and chelerythryn. However, under these conditions TPA stimulated HTLV-I LTR to a lesser extent than did the long-term TPA pretreatment. Moreover, this stimulation was enhanced by the PKC inhibitors. Thus our data indicate that while the effect of TPA on HIV-1 LTR is strictly dependent on PKC activity, its effect on HTLV-I LTR is exerted via a different pathway that not only does not require PKC activ...Continue Reading

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Citations

Aug 18, 2004·Retrovirology·Inbal AzranMordechai Aboud
Mar 29, 2011·Antiviral Research·Jenny Shvarzbeyn, Mahmoud Huleihel
Dec 5, 2008·Leukemia Research·Mahmoud EgbariaMahmoud Huleihel
Oct 6, 2007·International Journal of Cancer. Journal International Du Cancer·Yana Schavinsky-KhrapunskyMordechai Aboud
May 3, 2003·Biochemical and Biophysical Research Communications·Y Schavinsky-KhrapunskyA Torgeman
Jun 21, 2005·Virology·Marie-Eve ParéBenoit Barbeau
Mar 9, 2005·Immunology Letters·Zoltán BánkiManfred P Dierich
Jun 9, 2017·Cell Cycle·Azhar JabareenMahmoud Huleihel
Jan 10, 2009·PLoS Pathogens·John C BurnettDavid V Schaffer

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