The long-wavelength chlorophyll states of plant LHCI at room temperature: a comparison with PSI-LHCI

Biophysical Journal
Robert C JenningsFlavio M Garlaschi

Abstract

The red antenna states of the external antenna complexes of higher plant photosystem I, known as LHCI, have been analyzed by measurement of their preequilibrium fluorescence upon direct excitation at 280 K. In addition to the previously detected F735 state, a hitherto undetected low-energy state with emission maximum around 713 nm was observed. The 280 K bandwidths (FWHM) are 55 nm for the F735 state and approximately 27 nm for the F713-nm state, much greater than for non-red-shifted antenna chlorophylls. The origin absorption band for the F735-nm state was directly detected by determination of its excitation (action) spectrum and lies at 709-710 nm. The absorption spectrum for F735, calculated using the Stepanov expression, closely overlaps the excitation spectrum, indicating that the very large Stokes shift (25 nm) is due to vibrational relaxation within the excited-state manifold and solvent effects can be excluded. Fluorescence anisotropy measurements, with direct excitation of F735, indicate that the transition dipoles of the two red states are parallel. Similar experiments performed in the long-wavelength absorbing tail of PSI-LHCI indicate the presence of emission state(s) that are red-shifted with respect to F735 of iso...Continue Reading

References

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Citations

Jun 7, 2006·Photosynthesis Research·Giorgio FortiGiuseppe Zucchelli
Feb 4, 2014·Plant Physiology and Biochemistry : PPB·E G MaksimovV Z Paschenko
Jan 29, 2008·Biophysical Journal·Chavdar SlavovAlfred R Holzwarth
Dec 14, 2011·Biochimica Et Biophysica Acta·Marc BrechtNavassard V Karapetyan
Sep 6, 2005·Biochimica Et Biophysica Acta·Robert C JenningsGiuseppe Zucchelli
Feb 15, 2015·Biochimica Et Biophysica Acta·Clotilde Le QuiniouRoberta Croce
Mar 1, 2011·Biophysical Journal·Emilie WientjesRoberta Croce
Feb 8, 2005·Biochimica Et Biophysica Acta·Enrico C M EngelmannRobert C Jennings
Oct 16, 2016·Biochimica Et Biophysica Acta. Bioenergetics·Erica BelgioOndřej Prášil
Mar 21, 2017·Physical Chemistry Chemical Physics : PCCP·Stefano SantabarbaraStefano Caffarri
Apr 7, 2005·FEBS Letters·Giuseppe ZucchelliRobert C Jennings
Jun 16, 2018·Science·Dennis J NürnbergA William Rutherford
Jul 16, 2013·The Journal of Physical Chemistry. B·Joris J SnellenburgIvo H M van Stokkum
Jul 21, 2006·The Journal of Physical Chemistry. B·Janne A IhalainenRienk van Grondelle

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